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Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters.

Authors :
Petit V
Massonnet G
Maciorowski Z
Touhami J
Thuleau A
Némati F
Laval J
Château-Joubert S
Servely JL
Vallerand D
Fontaine JJ
Taylor N
Battini JL
Sitbon M
Decaudin D
Source :
Laboratory investigation; a journal of technical methods and pathology [Lab Invest] 2013 May; Vol. 93 (5), pp. 611-21. Date of Electronic Publication: 2013 Mar 04.
Publication Year :
2013

Abstract

Metabolic adaptations and changes in the expression of nutrient transporters are known to accompany tumorigenic processes. Nevertheless, in the context of solid tumors, studies of metabolism are hindered by a paucity of tools allowing the identification of cell surface transporters on individual cells. Here, we developed a method for the dissociation of human breast cancer tumor xenografts combined with quantification of cell surface markers, including metabolite transporters. The expression profiles of four relevant nutrient transporters for cancer cells' metabolism, Glut1, ASCT2, PiT1 and PiT2 (participating to glucose, glutamine and inorganic phosphate, respectively), as detected by new retroviral envelope glycoprotein-derived ligands, were distinctive of each tumor, unveiling underlying differences in metabolic pathways. Our tumor dissociation procedure and nutrient transporter profiling technology provides opportunities for future basic research, clinical diagnosis, prognosis and evaluation of therapeutic responses, as well as for drug discovery and development.

Details

Language :
English
ISSN :
1530-0307
Volume :
93
Issue :
5
Database :
MEDLINE
Journal :
Laboratory investigation; a journal of technical methods and pathology
Publication Type :
Academic Journal
Accession number :
23459372
Full Text :
https://doi.org/10.1038/labinvest.2013.44