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Immune adjuvant effect of V. cholerae O1 derived Proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi.
- Source :
-
BMC immunology [BMC Immunol] 2013; Vol. 14 Suppl 1, pp. S10. Date of Electronic Publication: 2013 Feb 25. - Publication Year :
- 2013
-
Abstract
- The proteoliposome derived from Vibrio cholerae O1 (PLc) is a nanoscaled structure obtained by a detergent extraction process. Intranasal (i.n) administration of PLc was immunogenic at mucosal and systemic level vs. V. cholerae; however the adjuvant potential of this structure for non-cholera antigens has not been proven yet. The aim of this work was to evaluate the effect of coadministering PLc with the Vi polysaccharide antigen (Poli Vi) of S. Typhi by the i.n route. The results showed that Poli Vi coadministered with PLc (PLc+Poli Vi) induce a higher IgA response in saliva (p<0.01) and faeces (p<0.01) than Poli Vi administered alone. Likewise, the IgG response in sera was higher in animals immunised with PLc+Poli Vi (p<0.01). Furthermore, IgG induced in sera of mice immunised with PLc+Poli Vi was similar (p>0.05) to that induced in a group of mice immunised by the parenteral route with the Cuban anti-typhoid vaccine vax-TyVi, although this vaccine did not induce a mucosal response. In conclusion, this work demonstrates that PLc can be used as a mucosal adjuvant to potentiate the immune response against a polysaccharide antigen like Poli Vi.
- Subjects :
- Administration, Intranasal
Animals
Antibodies, Bacterial blood
Antibodies, Bacterial immunology
Feces
Female
Immunoglobulin A immunology
Immunoglobulin G blood
Immunoglobulin G immunology
Mice
Mice, Inbred BALB C
Polysaccharides, Bacterial administration & dosage
Proteolipids administration & dosage
Saliva immunology
Typhoid Fever prevention & control
Typhoid-Paratyphoid Vaccines administration & dosage
Adjuvants, Immunologic administration & dosage
Polysaccharides, Bacterial immunology
Proteolipids immunology
Salmonella typhi immunology
Typhoid Fever immunology
Typhoid-Paratyphoid Vaccines immunology
Vibrio cholerae O1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2172
- Volume :
- 14 Suppl 1
- Database :
- MEDLINE
- Journal :
- BMC immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23458379
- Full Text :
- https://doi.org/10.1186/1471-2172-14-S1-S10