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Protective roles of methionine-R-sulfoxide reductase against stresses in Schizosaccharomyces pombe.
- Source :
-
Journal of basic microbiology [J Basic Microbiol] 2014 Jan; Vol. 54 (1), pp. 72-80. Date of Electronic Publication: 2013 Mar 01. - Publication Year :
- 2014
-
Abstract
- The Schizosaccharomyces pombe msrB(+) gene encoding methionine-R-sulfoxide reductase (MsrB) was cloned into the shuttle vector pRS316 to generate the recombinant plasmid pFMetSO. The msrB(+) mRNA level was significantly increased in the S. pombe cells harboring pFMetSO, indicating that the cloned msrB(+) gene is functioning. In the presence of 0.1 mM L-methionine-(R,S)-sulfoxide, the S. pombe cells harboring pFMetSO could grow normally but the growth of the vector control cells was almost arrested. The S. pombe cells harboring pFMetSO exhibited the enhanced growth on the minimal medium plates with stress-inducing agents, such as hydrogen peroxide, superoxide radical-generating menadione (MD), nitric oxide (NO)-generating sodium nitroprusside (SNP), and cadmium (Cd), when compared with the vector control cells. They also gave rise to the enhanced growth at the high incubation temperature of 37 °C than the vector control cells. The S. pombe cells harboring pFMetSO contained lower reactive oxygen species (ROS) and higher total glutathione (GSH) levels than the vector control cells. In brief, the S. pombe MsrB plays a protective role against oxidative, nitrosative, and thermal stresses, and is involved in diminishing intracellular ROS level.<br /> (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Amino Acid Sequence
Base Sequence
Glutathione metabolism
Methionine Sulfoxide Reductases genetics
Molecular Sequence Data
Reactive Oxygen Species metabolism
Schizosaccharomyces genetics
Schizosaccharomyces growth & development
Stress, Physiological
Methionine Sulfoxide Reductases metabolism
Schizosaccharomyces physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4028
- Volume :
- 54
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of basic microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 23456650
- Full Text :
- https://doi.org/10.1002/jobm.201200397