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Pneumonia-induced sepsis in mice: temporal study of inflammatory and cardiovascular parameters.
- Source :
-
International journal of experimental pathology [Int J Exp Pathol] 2013 Apr; Vol. 94 (2), pp. 144-55. Date of Electronic Publication: 2013 Feb 27. - Publication Year :
- 2013
-
Abstract
- The aim of the present work is to provide a better comprehension of the pneumonia-induced sepsis model through temporal evaluation of several parameters, and thus identify the main factors that determine mortality in this model. Klebsiella pneumoniae was inoculated intratracheally in anesthetized Swiss male mice. Inflammatory and cardiovascular parameters were evaluated 6, 24 and 48 h after the insult. The results show that severity of infection and the mortality correlated with the amount of bacteria. Six, 24 and 48 h after inoculation, animals presented pathological changes in lungs, increase in cell number in the bronchoalveolar lavage, leukopenia, increase in TNF-α and IL-1β levels, hypotension and hyporesponsiveness to vasoconstrictors, the two latter characteristics of severe sepsis and septic shock. Significant numbers of bacteria in spleen and heart homogenates indicated infection spreading. Interestingly, NOS-2 expression appeared late after bacteria inoculation, whereas levels of NOS-1 and NOS-3 were unchanged. The high NOS-2 expression coincided with an exacerbated NO production in the infection focus and in plasma, as judging by nitrate + nitrite levels. This study shows that K. pneumoniae inoculation induces a systemic inflammatory response and cardiovascular alterations, which endures at least until 48 h. K. pneumoniae-induced lung infection is a clinically relevant animal model of sepsis and a better understanding of this model may help to increase the knowledge about sepsis pathophysiology.<br /> (© 2013 The Authors. International Journal of Experimental Pathology © 2013 International Journal of Experimental Pathology.)
- Subjects :
- Animals
Bacterial Load
Bronchoalveolar Lavage Fluid
Cytokines metabolism
Disease Models, Animal
Humans
Klebsiella Infections immunology
Klebsiella Infections mortality
Leukocytes
Lung immunology
Lung metabolism
Male
Mice
Myocardium immunology
Myocardium metabolism
Nitrates metabolism
Nitric Oxide metabolism
Nitric Oxide Synthase Type II metabolism
Nitrites metabolism
Pneumonia, Bacterial immunology
Pneumonia, Bacterial microbiology
Pneumonia, Bacterial mortality
Sepsis immunology
Sepsis microbiology
Sepsis mortality
Spleen immunology
Spleen metabolism
Klebsiella Infections pathology
Klebsiella pneumoniae immunology
Pneumonia, Bacterial pathology
Sepsis pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2613
- Volume :
- 94
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 23441627
- Full Text :
- https://doi.org/10.1111/iep.12016