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Adiponectin increases glucose-induced insulin secretion through the activation of lipid oxidation.
- Source :
-
Acta diabetologica [Acta Diabetol] 2013 Dec; Vol. 50 (6), pp. 851-7. Date of Electronic Publication: 2013 Feb 27. - Publication Year :
- 2013
-
Abstract
- The expression of adiponectin receptors has been demonstrated in human and rat pancreatic beta cells, where globular (g) adiponectin rescues rat beta cells from cytokine and fatty acid-induced apoptosis. The aim of our study was to evaluate whether adiponectin has a direct effect on insulin secretion and the metabolic pathways involved. Purified human pancreatic islets and rat beta cells (INS-1E) were exposed (1 h) to g-adiponectin, and glucose-induced insulin secretion was measured. A significant increase in glucose-induced insulin secretion was observed in the presence of g-adiponectin (1 nmol/l) with respect to control cells in both human pancreatic islets (n = 5, p < 0.05) and INS-1E cells (n = 5, p < 0.001). The effect of globular adiponectin on insulin secretion was independent of AMP-dependent protein kinase (AMPK) activation or glucose oxidation. In contrast, g-adiponectin significantly increased oleate oxidation (n = 5, p < 0.05), and the effect of g-adiponectin (p < 0.001) on insulin secretion by INS-1E was significantly reduced in the presence of etomoxir (1 μmol/l), an inhibitor of fatty acid beta oxidation. g-Adiponectin potentiates glucose-induced insulin secretion in both human pancreatic islets and rat beta cells via an AMPK independent pathway. Increased fatty acid oxidation rather than augmented glucose oxidation is the mechanism responsible. Overall, our data indicate that, in addition to its anti-apoptotic action, g-adiponectin has another direct effect on beta cells by potentiating insulin secretion. Adiponectin, therefore, in addition to its well-known effect on insulin sensitivity, has important effects at the pancreatic level.
- Subjects :
- Adenylate Kinase metabolism
Animals
Fatty Acids metabolism
Humans
Insulin Secretion
Insulin-Secreting Cells drug effects
Insulin-Secreting Cells metabolism
Islets of Langerhans drug effects
Islets of Langerhans metabolism
Oxidation-Reduction drug effects
Rats
Tumor Cells, Cultured
Adiponectin pharmacology
Glucose pharmacology
Insulin metabolism
Lipid Metabolism drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1432-5233
- Volume :
- 50
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Acta diabetologica
- Publication Type :
- Academic Journal
- Accession number :
- 23440352
- Full Text :
- https://doi.org/10.1007/s00592-013-0458-x