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Enhancing post-expansion chondrogenic potential of costochondral cells in self-assembled neocartilage.
- Source :
-
PloS one [PLoS One] 2013; Vol. 8 (2), pp. e56983. Date of Electronic Publication: 2013 Feb 21. - Publication Year :
- 2013
-
Abstract
- The insufficient healing capacity of articular cartilage necessitates mechanically functional biologic tissue replacements. Using cells to form biomimetic cartilage implants is met with the challenges of cell scarcity and donor site morbidity, requiring expanded cells that possess the ability to generate robust neocartilage. To address this, this study assesses the effects of expansion medium supplementation (bFGF, TFP, FBS) and self-assembled construct seeding density (2, 3, 4 million cells/5 mm dia. construct) on the ability of costochondral cells to generate biochemically and biomechanically robust neocartilage. Results show TFP (1 ng/mL TGF-β1, 5 ng/mL bFGF, 10 ng/mL PDGF) supplementation of serum-free chondrogenic expansion medium enhances the post-expansion chondrogenic potential of costochondral cells, evidenced by increased glycosaminoglycan content, decreased type I/II collagen ratio, and enhanced compressive properties. Low density (2 million cells/construct) enhances matrix synthesis and tensile and compressive mechanical properties. Combined, TFP and Low density interact to further enhance construct properties. That is, with TFP, Low density increases type II collagen content by over 100%, tensile stiffness by over 300%, and compressive moduli by over 140%, compared with High density. In conclusion, the interaction of TFP and Low density seeding enhances construct material properties, allowing for a mechanically functional, biomimetic cartilage to be formed using clinically relevant costochondral cells.
- Subjects :
- Animals
Biomechanical Phenomena
Cartilage, Articular chemistry
Cartilage, Articular physiology
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Collagen Type II metabolism
Glycosaminoglycans metabolism
Swine
Cartilage, Articular cytology
Chondrocytes cytology
Chondrogenesis physiology
Tissue Engineering
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23437288
- Full Text :
- https://doi.org/10.1371/journal.pone.0056983