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Protective effect of combined therapy with dithiothreitol, zinc and selenium protects acute mercury induced oxidative injury in rats.
- Source :
-
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2013 Jul; Vol. 27 (3), pp. 249-56. Date of Electronic Publication: 2013 Feb 19. - Publication Year :
- 2013
-
Abstract
- The present study was undertaken to establish mode of action, comparative therapeutic efficacy and safety evaluation of dithiothreitol (DTT) supplemented with Zn and Se against dimethylmercury in rats. Adult male albino rats of Sprague-Dawley strain (150±10 g, n=6 per group) were exposed a bolus dose of dimethylmercury (10 mg/kg, p.o.) for once only followed by DTT (15.4 mg/kg, i.p.) along with the combination of antioxidants Zn and Se (2 mmol/kg and 0.5 mg/kg, p.o.) after 72 h of toxicant administration for three days. The results showed a significant (P≤0.05) increase in the activities of AST, ALT, alkaline phosphatase, lactate dehydrogenase, in serum after toxicant administration. This was accompanied by histopathological observations. A significant rise was observed in lipid peroxidation level and mercury ion concentration however reduced glutathione content decreased in liver, kidney and brain. A significant (P≤0.05) decrease in the activity of acetyl cholinesterase was also seen in different regions of brain. Combined treatment of DTT along with Zn and Se significantly (P≤0.05) recouped the alterations in the enzymatic activities of serum and reversed the tissue biochemical and histopathological changes of liver, kidney and brain. Our results demonstrate that combined treatment of thiol chelator (DTT) along with antioxidants (Zn and Se) plays an important role against dimethylmercury induced tissue damage and hepatic, nephro and neurotoxicity.<br /> (Copyright © 2013. Published by Elsevier GmbH.)
- Subjects :
- Animals
Antioxidants administration & dosage
Antioxidants pharmacology
Brain drug effects
Brain metabolism
Brain pathology
Chelating Agents administration & dosage
Chelating Agents pharmacology
Combined Modality Therapy
Dithiothreitol administration & dosage
Kidney drug effects
Kidney metabolism
Kidney pathology
Liver drug effects
Liver metabolism
Liver pathology
Male
Methylmercury Compounds administration & dosage
Rats
Rats, Sprague-Dawley
Sodium Selenite administration & dosage
Zinc Acetate administration & dosage
Cytoprotection drug effects
Dithiothreitol pharmacology
Methylmercury Compounds toxicity
Oxidative Stress drug effects
Sodium Selenite pharmacology
Zinc Acetate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3252
- Volume :
- 27
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)
- Publication Type :
- Academic Journal
- Accession number :
- 23428462
- Full Text :
- https://doi.org/10.1016/j.jtemb.2012.12.003