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Cyclic stretch augments production of neutrophil chemokines and matrix metalloproteinases-1 (MMP-1) from human decidual cells, and the production was reduced by progesterone.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2013 May; Vol. 69 (5), pp. 454-62. Date of Electronic Publication: 2013 Feb 19. - Publication Year :
- 2013
-
Abstract
- Problem: The purpose of this study was to evaluate the impact of mechanical stretch caused by uterine contraction and progesterone (P₄) on decidual cells (DC), neutrophil chemokines, and MMP-1 expression.<br />Method of Study: DC were cultured, and cyclic stretch was applied using a computer-operated cell stretch system. Interleukin (IL)-8, growth-regulated oncogene (GRO) α, matrix metalloproteinase (MMP)-1, and mRNA and/or protein expression/activity was measured using RT-PCR and ELISA. Neutrophil chemotactic activity in conditioned media was evaluated using migration assays. The effect of P₄ was also studied.<br />Results: Cyclic mechanical stretch increased IL-8, GROα, mRNA and protein, and MMP-1 production and activity level. Supernatant from stretched cells induced neutrophil chemotactic activity significantly. P₄ suppressed the effect of stretch.<br />Conclusion: The current study demonstrates that cyclic mechanical stretch stimulates the production of neutrophil chemokines and MMP-1 from human decidual cells, and the production was reduced by progesterone. These findings suggest that decidual cells are responding to mechanical and endocrine signals and induce biochemical factors and thereby contribute to the regulation of human labor.<br /> (© 2013 John Wiley & Sons A/S.)
- Subjects :
- Cells, Cultured
Chemokine CXCL1 genetics
Chemokine CXCL1 metabolism
Chemokines metabolism
Culture Media, Conditioned pharmacology
Decidua cytology
Decidua drug effects
Female
Gene Expression Regulation drug effects
Gene Expression Regulation immunology
Humans
Interleukin-8 genetics
Interleukin-8 metabolism
Matrix Metalloproteinase 1 genetics
Mechanotransduction, Cellular drug effects
Neutrophils drug effects
Progesterone pharmacology
RNA, Messenger analysis
Decidua metabolism
Matrix Metalloproteinase 1 metabolism
Mechanotransduction, Cellular immunology
Neutrophils metabolism
Uterine Contraction immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0897
- Volume :
- 69
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 23418737
- Full Text :
- https://doi.org/10.1111/aji.12092