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Dynamics of the striped bass (Morone saxatilis) ovary proteome reveal a complex network of the translasome.
- Source :
-
Journal of proteome research [J Proteome Res] 2013 Apr 05; Vol. 12 (4), pp. 1691-9. Date of Electronic Publication: 2013 Mar 04. - Publication Year :
- 2013
-
Abstract
- We evaluated changes in the striped bass (Morone saxatilis) ovary proteome during the annual reproductive cycle using label-free quantitative mass spectrometry and a novel machine learning analysis based on K-means clustering and support vector machines. Modulated modularity clustering was used to group co-variable proteins into expression modules and Gene Ontology (GO) biological process and KEGG pathway enrichment analyses were conducted for proteins within those modules. We discovered that components of the ribosome along with translation initiation and elongation factors generally decrease as the annual ovarian cycle progresses toward ovulation, concomitant with a slight increase in components of the 26S-proteasome. Co-variation within more than one expression module of components from these two multi-protein complexes suggests that they are not only co-regulated, but that co-regulation occurs through more than one sub-network. These components also co-vary with subunits of the TCP-1 chaperonin system and enzymes of intermediary metabolic pathways, suggesting that protein folding and cellular bioenergetic state play important roles in protein synthesis and degradation. We provide further evidence to suggest that protein synthesis and degradation are intimately linked, and our results support function of a proteasome-ribosome supercomplex known as the translasome.
- Subjects :
- Animals
Artificial Intelligence
Bass
Cluster Analysis
Female
Fish Proteins genetics
Gene Ontology
Mass Spectrometry methods
Proteasome Endopeptidase Complex metabolism
Ribosomes genetics
Ribosomes metabolism
Fish Proteins metabolism
Menstrual Cycle physiology
Ovary metabolism
Proteome metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 23414552
- Full Text :
- https://doi.org/10.1021/pr3010293