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The effect of synthetic C-reactive protein on the in vitro immune response of human PBMCs stimulated with bacterial reagents.
- Source :
-
Inflammation [Inflammation] 2013 Aug; Vol. 36 (4), pp. 781-92. - Publication Year :
- 2013
-
Abstract
- Synthetic C-reactive protein (CRP) rescues mice from lethal endotoxin shock or bacterial infection by suppressing tumor necrosis factor (TNF-α), but in turn, enhances Kupffer cell phagocytic activity. We herein assessed the influence of CRP in human peripheral blood mononuclear cells (PBMCs). When human PBMCs were stimulated in vitro with penicillin-treated Streptococcus pyogenes, bacterial DNA motifs and lipopolysaccharide with or without synthetic CRP, CRP suppressed the production of TNF-α and IL-12, but not that of IFN-γ. This was also the case for the in vitro Shwartzman reaction induced in PBMCs. CRP also decreased high-mobility group box 1 production from macrophages, which is crucial in the later phase of endotoxin/septic shock. However, CRP upregulated the perforin expression by CD56(+) NK cells and increased their antitumor cytotoxicity. CRP may thus be a potent immunomodulatory factor in the human immune system, suggesting its therapeutic potential for use against human septic shock.
- Subjects :
- Animals
C-Reactive Protein therapeutic use
DNA, Bacterial immunology
HMGB1 Protein biosynthesis
HMGB1 Protein immunology
Humans
Interferon-gamma biosynthesis
Interferon-gamma immunology
Interleukin-12 biosynthesis
Interleukin-12 immunology
Killer Cells, Natural immunology
Killer Cells, Natural metabolism
Kupffer Cells immunology
Kupffer Cells metabolism
Leukocytes, Mononuclear immunology
Lipopolysaccharides immunology
Macrophages drug effects
Macrophages immunology
Macrophages metabolism
Mice
Perforin biosynthesis
Perforin immunology
Recombinant Proteins pharmacology
Shwartzman Phenomenon immunology
Tumor Necrosis Factor-alpha biosynthesis
Tumor Necrosis Factor-alpha immunology
C-Reactive Protein pharmacology
Leukocytes, Mononuclear drug effects
Shock, Septic drug therapy
Streptococcus pyogenes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-2576
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 23407995
- Full Text :
- https://doi.org/10.1007/s10753-013-9604-4