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Distribution of pejvakin in human spiral ganglion: An immunohistochemical study.
- Source :
-
Cochlear implants international [Cochlear Implants Int] 2013 Sep; Vol. 14 (4), pp. 225-31. Date of Electronic Publication: 2013 Feb 12. - Publication Year :
- 2013
-
Abstract
- Up to 10% of permanent hearing impairments in children originate from lesions in the neuronal auditory pathway. This form of auditory neuron injury called auditory neuropathy features a preservation of outer hair cell integrity but an impaired inner hair cell function and/or neuronal transmission. DFNB59 gene encodes the protein pejvakin (PJVK) and its mutations cause autosomal recessive auditory neuropathy as well as other forms of sensorineural hearing loss. The finding of distinct forms of hearing anomalies was based on studies of consanguineous families from different ethnic groups as well as studies in mice with PJVK gene mutations. In the present immunohistochemical study, the distribution of pejvakin protein in surgically obtained human cochleae was for the first time investigated. The human cochleae had normal hearing thresholds before the operation. The expression of pejvakin was located in the cell bodies of all spiral ganglion neurons rather than the nerve fibers that were labeled with Tuj 1 antibody. As Tuj 1 antibody stained the cytoplasm of Type 1 cells, pejvakin antibody labeled both type 1 and type 2 cells. The nuclei of the neurons were also PJVK-positive. No labeling was seen in the structures within the organ of Corti and the stria vascularis. In the previous study, PJVK had been detected in the hair cells, the spiral ganglion, the cochlear nuclei, the superior olivary nucleus, and the inferior colliculus in mouse. Our study demonstrated for the first time the expression of PJVK in human spiral ganglion neurons. Its functional role in neural signal propagation and synchrony needs further elucidation.
- Subjects :
- Adult
Female
Hair Cells, Auditory, Inner pathology
Hair Cells, Auditory, Inner ultrastructure
Hearing Loss, Sensorineural genetics
Humans
Immunohistochemistry
Male
Microscopy, Confocal
Middle Aged
Reference Values
Sampling Studies
Tissue Culture Techniques
Auditory Pathways pathology
Gene Expression Regulation
Nerve Tissue Proteins genetics
Spiral Ganglion pathology
Spiral Ganglion ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1754-7628
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cochlear implants international
- Publication Type :
- Academic Journal
- Accession number :
- 23407324
- Full Text :
- https://doi.org/10.1179/1754762812Y.0000000027