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[Primers for (1,3)-β-glucan synthase gene amplification and partial characterization of the enzyme in Ganoderma lucidum].
- Source :
-
Revista iberoamericana de micologia [Rev Iberoam Micol] 2013 Oct-Dec; Vol. 30 (4), pp. 267-70. Date of Electronic Publication: 2013 Feb 09. - Publication Year :
- 2013
-
Abstract
- Background: β-(1,3)(1,6)-D-glucan is fungal cell wall component that has demonstrated immunomodulatory and anti-cancer effects. The (1,3)-β-glucan synthase is one of the main enzymes involved in its biosynthesis.<br />Aims: To design primers to partially amplify and characterize the (1,3)-β-glucan synthase gene and to determine them in Ganoderma lucidum (G. Lucidum) strain CP-132.<br />Methods: The primers were designed on the basis of homologous genes in other fungi. Then, using the PCR technique, primers were tested using DNA extracted from the G. lucidum strain CP-382. Amplified sequences were compared with those from the GenBank.<br />Results: Three primer pairs were designed; all of them produced amplicons of the expected size. The sequences obtained with primer pairs BGS2113UmF and BGS3097UmR, and BGS547UmF and BGS2113UmR matched with 2 sections of the (1,3)-β-glucan synthase gene. The deduced amino acid sequences showed high similarity with homologous genes from other fungi, particularly with those of the Agaricomycetes class.<br />Conclusions: The primer design to partially amplify the (1,3)-β-glucan synthase gene of G. lucidum using sequences from homologous genes was successful. These primers will allow to characterize this important enzyme in a wide group of fungi.<br /> (Copyright © 2012 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.)
- Subjects :
- Agaricales enzymology
Agaricales genetics
Amino Acid Sequence
Base Sequence
DNA Primers
DNA, Intergenic genetics
Molecular Sequence Data
Molecular Weight
Phylogeny
Reishi genetics
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
DNA, Fungal genetics
Fungal Proteins genetics
Glucosyltransferases genetics
Polymerase Chain Reaction methods
Reishi enzymology
Subjects
Details
- Language :
- Spanish; Castilian
- ISSN :
- 2173-9188
- Volume :
- 30
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Revista iberoamericana de micologia
- Publication Type :
- Academic Journal
- Accession number :
- 23402832
- Full Text :
- https://doi.org/10.1016/j.riam.2012.12.006