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Phosphate limitation induces the intergeneric inhibition of Pseudomonas aeruginosa by Serratia marcescens isolated from paper machines.
- Source :
-
FEMS microbiology ecology [FEMS Microbiol Ecol] 2013 Jun; Vol. 84 (3), pp. 577-87. Date of Electronic Publication: 2013 Mar 11. - Publication Year :
- 2013
-
Abstract
- Phosphate is an essential nutrient for heterotrophic bacteria, affecting bacterioplankton in aquatic ecosystems and bacteria in biofilms. However, the influence of phosphate limitation on bacterial competition and biofilm development in multispecies populations has received limited attention in existing studies. To address this issue, we isolated 13 adhesive bacteria from paper machine aggregates. Intergeneric inhibition of Pseudomonas aeruginosa WW5 by Serratia marcescens WW4 was identified under phosphate-limited conditions, but not in Luria-Bertani medium or M9 minimal medium. The viable numbers of the pure S. marcescens WW4 culture decreased over 3 days in the phosphate-limited medium; however, the mortality of S. marcescens WW4 was significantly reduced when it was co-cultured with P. aeruginosa WW5, which appeared to sustain the S. marcescens WW4 biofilm. In contrast, viable P. aeruginosa WW5 cells immediately declined in the phosphate-limited co-culture. To identify the genetic/inhibitory element(s) involved in this process, we inserted a mini-Tn5 mutant of S. marcescens WW4 that lacked inhibitory effect. The results showed that an endonuclease bacteriocin was involved in this intergeneric inhibition by S. marcescens WW4 under phosphate limitation. In conclusion, this study highlights the importance of nutrient limitation in bacterial interactions and provides a strong candidate gene for future functional characterisation.<br /> (© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.)
- Subjects :
- Antibiosis
Bacterial Adhesion
Bacteriocins metabolism
Biofilms drug effects
Culture Media
DNA Transposable Elements
Microbial Viability
Mutagenesis, Insertional
Paper
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa isolation & purification
Serratia marcescens drug effects
Serratia marcescens genetics
Serratia marcescens isolation & purification
Biofilms growth & development
Microbial Interactions
Phosphates pharmacology
Pseudomonas aeruginosa physiology
Serratia marcescens physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1574-6941
- Volume :
- 84
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEMS microbiology ecology
- Publication Type :
- Academic Journal
- Accession number :
- 23398522
- Full Text :
- https://doi.org/10.1111/1574-6941.12086