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Assessment of DNA damage by micellar electrokinetic chromatography.

Authors :
Fattorini P
Marrubini G
Grignani P
Sorçaburu-Cigliero S
Previderé C
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 984, pp. 341-51.
Publication Year :
2013

Abstract

A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of uncanonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR templates used in several fields of investigation.

Subjects

Subjects :
Buffers
Calibration
Chromatography, Micellar Electrokinetic Capillary methods
Chromatography, Micellar Electrokinetic Capillary standards
DNA chemistry
DNA isolation & purification
Electrophoresis, Capillary methods
Forensic Genetics
Humans
Hydrolysis
Limit of Detection
Polymerase Chain Reaction
Reference Standards
Uridine Triphosphate analogs & derivatives
Uridine Triphosphate chemistry
DNA Damage

Details

Language :
English
ISSN :
1940-6029
Volume :
984
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
23386356
Full Text :
https://doi.org/10.1007/978-1-62703-296-4_25
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