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West Nile virus in mosquitoes in Greece.
- Source :
-
Parasitology research [Parasitol Res] 2013 Apr; Vol. 112 (4), pp. 1551-5. Date of Electronic Publication: 2013 Jan 31. - Publication Year :
- 2013
-
Abstract
- Epidemics of West Nile virus (WNV) occurred for two consecutive years in Greece (in 2010 and 2011). A total of 16,116 adult Culex pipiens mosquitoes collected in two peripheries, Central Macedonia and Thessaly, were tested for WNV infection. WNV lineage 2 was detected in 6/296 mosquito pools, three in each year. The H249P substitution in the NS3 protein, previously associated with increased pathogenicity and thermotolerance, was detected in all six WNV-positive mosquito pools. When 21 individual C. pipiens mosquitoes were tested for the locus CQ11 to distinguish between the two C. pipiens forms, pipiens and molestus, 71.4% were identified as pipiens, 4.7% as molestus, and 19% as hybrid pipiens/molestus, giving the first evidence that both C. pipiens biotypes are present in Greece, with a significant proportion being hybrids. The exact role of the C. pipiens forms and hybrids in the WNV epidemiology, in combination or not with the H249P substitution in the virus genome, remains to be elucidated.
- Subjects :
- Amino Acid Substitution
Animals
Culex classification
Culex genetics
Greece
Mutation, Missense
Polymerase Chain Reaction
Sequence Analysis, DNA
Viral Nonstructural Proteins genetics
West Nile virus classification
West Nile virus genetics
Culex virology
Genetic Variation
West Nile virus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1955
- Volume :
- 112
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 23371497
- Full Text :
- https://doi.org/10.1007/s00436-013-3302-x