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Prognostic significance of combinations of RNA-dependent protein kinase and EphA2 biomarkers for NSCLC.
- Source :
-
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer [J Thorac Oncol] 2013 Mar; Vol. 8 (3), pp. 301-8. - Publication Year :
- 2013
-
Abstract
- Introduction: RNA-dependent protein kinase (PKR) is an independent prognostic variable in patients with non-small-cell lung cancer (NSCLC). In the current study, we investigated the correlation between PKR and 25 other biomarkers for NSCLC, identified the markers that could further improve the prognostic significance of PKR and elucidated the mechanisms of interaction between these markers and PKR.<br />Methods: Tissue microarray samples obtained from 218 patients with lung cancer were stained with an anti-PKR antibody and antibodies against 25 biomarkers. Immunohistochemical expression was scored and used for Kaplan-Meier survival analysis. The interaction between PKR and EphA2 in NSCLC cell lines was examined.<br />Results: We found that PKR was associated with EphA2 and that the prognostic information regarding NSCLC provided by the combination of PKR and EphA2 (P/E) was significantly more accurate than that provided by either marker alone. The 5-year overall survival rate in patients with PKR/EphA2 (20%) was significantly lower than that of patients with PKR/EphA2 (74%), patients with PKR/EphA2 (55%), and patients with PKR/EphA2 (55%) (p < 0.0001). We also found that the PKR:EphA2 (P/E) ratio was significantly associated with prognosis (p < 0.0001). Univariate and multivariate Cox analyses revealed that this P/E combination or ratio was an independent predictor of overall survival. In addition, induction of PKR expression reduced EphA2 protein expression levels in NSCLC cell lines.<br />Conclusions: PKR/EphA2 is a significant predictor of prognosis for NSCLC. PKR/EphA2 may be a promising approach to improving screening efficiency and predicting prognosis in patients with NSCLC.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Carcinoma, Non-Small-Cell Lung metabolism
Carcinoma, Non-Small-Cell Lung pathology
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Female
Follow-Up Studies
Humans
Immunoenzyme Techniques
Lung Neoplasms metabolism
Lung Neoplasms pathology
Male
Neoplasm Staging
Prognosis
RNA, Small Interfering genetics
Receptor, EphA2 antagonists & inhibitors
Receptor, EphA2 genetics
Survival Rate
Tissue Array Analysis
Tumor Cells, Cultured
Adenocarcinoma mortality
Biomarkers, Tumor metabolism
Carcinoma, Non-Small-Cell Lung mortality
Carcinoma, Squamous Cell mortality
Lung Neoplasms mortality
Receptor, EphA2 metabolism
eIF-2 Kinase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1556-1380
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 23370317
- Full Text :
- https://doi.org/10.1097/JTO.0b013e318282def7