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B cell receptor-induced Ca2+ mobilization mediates F-actin rearrangements and is indispensable for adhesion and spreading of B lymphocytes.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2013 Apr; Vol. 93 (4), pp. 537-47. Date of Electronic Publication: 2013 Jan 29. - Publication Year :
- 2013
-
Abstract
- B cells acquire membrane-bound cognate antigens from the surface of the APCs by forming an IS, similar to that seen in T cells. Recognition of membrane-bound antigens on the APCs initiates adhesion of B lymphocytes to the antigen-tethered surface, which is followed by the formation of radial lamellipodia-like structures, a process known as B cell spreading. The spreading response requires the rearrangement of the submembrane actin cytoskeleton and is regulated mainly via signals transmitted by the BCR. Here, we show that cytoplasmic calcium is a regulator of actin cytoskeleton dynamics in B lymphocytes. We find that BCR-induced calcium mobilization is indispensible for adhesion and spreading of B cells and that PLCγ and CRAC-mediated calcium mobilization are critical regulators of these processes. Measuring calcium and actin dynamics in live cells, we found that a generation of actin-based membrane protrusion is strongly linked to the dynamics of a cytoplasmic-free calcium level. Finally, we demonstrate that PLCγ and CRAC channels regulate the activity of actin-severing protein cofilin, linking BCR-induced calcium signaling to the actin dynamics.
- Subjects :
- Actin Cytoskeleton genetics
Actin Cytoskeleton immunology
Actins genetics
Actins immunology
Animals
Antigen Presentation
B-Lymphocytes cytology
B-Lymphocytes immunology
B-Lymphocytes metabolism
Calcium Channels genetics
Calcium Channels immunology
Cell Adhesion
Cell Line, Tumor
Cell Movement
Cofilin 1 genetics
Cofilin 1 immunology
Cofilin 1 metabolism
Gene Expression Regulation immunology
Genetic Vectors
Lentivirus genetics
Mice
Phospholipase C gamma genetics
Phospholipase C gamma immunology
Phospholipase C gamma metabolism
Pseudopodia immunology
Pseudopodia metabolism
Receptors, Antigen, B-Cell genetics
Receptors, Antigen, B-Cell immunology
Signal Transduction
Transduction, Genetic
Actin Cytoskeleton metabolism
Actins metabolism
Calcium metabolism
Calcium Channels metabolism
Receptors, Antigen, B-Cell metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1938-3673
- Volume :
- 93
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 23362305
- Full Text :
- https://doi.org/10.1189/jlb.0312169