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Identification of chicken calbindin D28K pre-messenger RNA sequences by polymerase chain reaction.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1990 Apr 30; Vol. 168 (2), pp. 430-6. - Publication Year :
- 1990
-
Abstract
- A transcribed RNA sequence encompassing the junction between the first intron and the second exon of the chicken calbindin D28K gene was copied in a cDNA fragment and subsequently amplified by polymerase chain reaction. When intestinal RNA is used as template, the appearance of the 161 bp amplified fragment is strictly dependent on the vitamin D status of the animal. In fact no amplified fragment is obtained when the RNA is extracted from the intestine of vitamin D-deficient chickens, while it is easily detected when the RNA is extracted only 30 min after injection with 1,25-dihydroxycholecalciferol. Conversely, the amplified fragment is obtained, irrespectively of the vitamin D status of the animal, when the RNA template is extracted from the brain. The appearance of unspliced RNA sequences upon vitamin D induction is followed, after a 30 min lag, by the appearance of the corresponding mature mRNA sequences.
- Subjects :
- Animals
Base Sequence
Brain Chemistry
Calbindins
Chickens
Intestines analysis
Molecular Sequence Data
Polymerase Chain Reaction
Transcription, Genetic
Vitamin D Deficiency genetics
Nucleic Acid Precursors analysis
RNA Processing, Post-Transcriptional
RNA, Messenger analysis
S100 Calcium Binding Protein G genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 168
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 2334414
- Full Text :
- https://doi.org/10.1016/0006-291x(90)92339-2