Back to Search
Start Over
Synovial perlecan is required for osteophyte formation in knee osteoarthritis.
- Source :
-
Matrix biology : journal of the International Society for Matrix Biology [Matrix Biol] 2013 Apr 24; Vol. 32 (3-4), pp. 178-87. Date of Electronic Publication: 2013 Jan 20. - Publication Year :
- 2013
-
Abstract
- The osteophyte associated with osteoarthritis (OA) is a bony outgrowth formed at the margins of the affected joint through endochondral ossification-like processes. However, the mechanism of osteophyte formation and its pathogenesis are unclear. Perlecan (Hspg2), a heparan sulfate proteoglycan, is expressed in many extracellular tissues and plays critical roles in skeletal development and diseases. The aim of the present study is to identify the role of synovial perlecan in osteophyte formation using perinatal lethality rescued perlecan-knockout mice (Hspg2(-/-)-Tg) wherein perlecan expression is lacking in the synovial and other tissues, except for cartilage. We analyzed the development of osteophytes in joints of Hspg2(-/-)-Tg mice in two different animal models: the surgical OA model, in which the medial collateral ligament was transected and the medial meniscus was resected, and the TGF-β-induced osteophyte formation model. In the surgical OA model, the osteophyte size and maturation were significantly reduced in the OA joints of Hspg2(-/-)-Tg mice compared with control mice, while OA developed on the medial side of the knee joints with no differences in the cartilage degradation score or synovitis score between control and Hspg2(-/-)-Tg mice. The reduced osteophyte formation in Hspg2(-/-)-Tg mice was associated with reduced cell proliferation and chondrogenesis. In the TGF-β model, the osteophyte size and maturation were also significantly reduced in Hspg2(-/-)-Tg mice compared with control mice. Our findings suggest that synovial perlecan plays an important role in osteophyte development in OA, and they provide insights that may facilitate the development of OA therapy.<br /> (Copyright © 2013 International Society of Matrix Biology. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cartilage, Articular metabolism
Cartilage, Articular pathology
Cell Differentiation
Cell Proliferation
Chondrocytes physiology
Female
Fibrillar Collagens metabolism
Heparan Sulfate Proteoglycans genetics
Mice
Mice, Knockout
Osteoarthritis, Knee pathology
Osteophyte pathology
Phosphorylation
Protein Processing, Post-Translational
Smad2 Protein metabolism
Transforming Growth Factor beta physiology
Heparan Sulfate Proteoglycans metabolism
Osteoarthritis, Knee metabolism
Osteophyte metabolism
Synovial Membrane metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1569-1802
- Volume :
- 32
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Matrix biology : journal of the International Society for Matrix Biology
- Publication Type :
- Academic Journal
- Accession number :
- 23339896
- Full Text :
- https://doi.org/10.1016/j.matbio.2013.01.004