Back to Search
Start Over
Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-coenzyme A: diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum.
- Source :
-
FEBS letters [FEBS Lett] 2013 Mar 01; Vol. 587 (5), pp. 481-7. Date of Electronic Publication: 2013 Jan 18. - Publication Year :
- 2013
-
Abstract
- Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body formation could be completely restored in a Saccharomyces cerevisiae TAG-deficient quadruple mutant by expressing PtDGAT2B. Up-regulation of PtDGAT2B precedes the accumulation of TAG. Functional analysis of enzyme activity in vivo demonstrated that expression of PtDGAT2B can increase the proportion of unsaturated C(16) and C(18) fatty acids in yeast TAG.<br /> (Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Motifs
Amino Acid Sequence
Conserved Sequence
Diacylglycerol O-Acyltransferase biosynthesis
Diacylglycerol O-Acyltransferase chemistry
Diacylglycerol O-Acyltransferase genetics
Diatoms metabolism
Gene Expression
Molecular Sequence Data
Phylogeny
Pichia
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae genetics
Substrate Specificity
Triglycerides biosynthesis
Triglycerides metabolism
Diatoms enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 587
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 23337871
- Full Text :
- https://doi.org/10.1016/j.febslet.2013.01.015