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Integrity of bacterial genomic DNA after autoclaving: possible implications for horizontal gene transfer and clinical waste management.
- Source :
-
The Journal of hospital infection [J Hosp Infect] 2013 Mar; Vol. 83 (3), pp. 247-9. Date of Electronic Publication: 2013 Jan 16. - Publication Year :
- 2013
-
Abstract
- Current autoclaving practices are designed to kill bacteria. Little is known about the effect of autoclaving on the integrity of bacterial genomic DNA. An experiment was performed to examine the effect of standard autoclaving on the integrity of bacterial DNA, employing polymerase chain reaction (PCR) as an indicator of DNA integrity. Amplifiable PCR signal was observed at t = 10, 20 and 30 min autoclaving time for Pseudomonas aeruginosa NCTC 10662; at t = 10, 20, 30 and 40 min for Salmonella Nottingham NCTC 7832; and at t = 10 and 20 min for Escherichia coli NCTC 9001. Careful consideration should therefore be given to residual molecular artefacts in future risk and environmental impact assessments, where the legacy of residual genomic DNA from dead bacterial and higher organisms may act as a potential reservoir, thereby feeding horizontal gene transfer scenarios in viable cells with potential hazardous genes of virulence, persistence or antibiotic resistance characteristics.<br /> (Copyright © 2012 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- DNA, Bacterial genetics
Escherichia coli radiation effects
Hot Temperature
Humans
Polymerase Chain Reaction
Pseudomonas aeruginosa radiation effects
Salmonella radiation effects
Time Factors
DNA, Bacterial radiation effects
Escherichia coli genetics
Gene Transfer, Horizontal radiation effects
Medical Waste Disposal methods
Pseudomonas aeruginosa genetics
Salmonella genetics
Sterilization methods
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2939
- Volume :
- 83
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of hospital infection
- Publication Type :
- Academic Journal
- Accession number :
- 23333146
- Full Text :
- https://doi.org/10.1016/j.jhin.2012.11.016