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Role of arachidonic acid metabolism in Stat5 activation induced by oleic acid in MDA-MB-231 breast cancer cells.
- Source :
-
Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2013 Mar; Vol. 88 (3), pp. 243-9. Date of Electronic Publication: 2013 Jan 17. - Publication Year :
- 2013
-
Abstract
- Epidemiological studies and animal models suggest an association between high levels of dietary fat intake and an increased risk of breast cancer. In breast cancer cells, the free fatty acid oleic acid (OLA) induces proliferation, migration, invasion and an increase of MMP-9 secretion. However, the role of OLA on Stat5 activation and the participation of COX-2 and LOXs activity in Stat5 activation induced by OLA remain to be investigated. We demonstrate here that stimulation of MDA-MB-231 breast cancer cells with 100 μM OLA induces Stat5 phosphorylation at Tyr-694 and an increase of Stat5-DNA complex formation. The Stat5 DNA-binding activity requires COX-2, LOXs, metalloproteinases and Src activities. In addition, OLA induces cell migration through a Stat5-dependent pathway. In summary, our findings establish that OLA induces cell migration through a Stat5-dependent pathway and that Stat5 activation requires AA metabolites in MDA-MB-231 breast cancer cells.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Breast Neoplasms
Cell Movement drug effects
Cyclooxygenase 2 metabolism
Dipeptides pharmacology
Electrophoretic Mobility Shift Assay
Female
Humans
Indoles pharmacology
Linoleic Acid pharmacology
Linoleic Acid physiology
Lipoxygenases metabolism
MCF-7 Cells
Matrix Metalloproteinase Inhibitors pharmacology
Matrix Metalloproteinases metabolism
Oleic Acid pharmacology
Protein Binding
Signal Transduction
Sulfonamides pharmacology
src-Family Kinases antagonists & inhibitors
src-Family Kinases metabolism
Arachidonic Acid metabolism
Oleic Acid physiology
STAT5 Transcription Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2823
- Volume :
- 88
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Prostaglandins, leukotrienes, and essential fatty acids
- Publication Type :
- Academic Journal
- Accession number :
- 23332799
- Full Text :
- https://doi.org/10.1016/j.plefa.2012.12.003