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Optimized immunohistochemistry in combination with image analysis: a reliable alternative to quantitative ELISA determination of uPA and PAI-1 for routine risk group discrimination in breast cancer.
- Source :
-
Breast (Edinburgh, Scotland) [Breast] 2013 Oct; Vol. 22 (5), pp. 736-43. Date of Electronic Publication: 2013 Jan 16. - Publication Year :
- 2013
-
Abstract
- The determination of the invasion markers urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) has further improved the possibilities for individualized therapy of breast cancer. To date, quantitative measurement by ELISA, that needs large amounts of fresh, frozen material, is the only standardized procedure for diagnostic purposes. Therefore, the aim of this study was the establishment of a reliable alternative method based on immunohistochemistry (IHC) and image analysis requiring only small amounts of fixed tumor tissue. Protein expression of uPA and PAI-1 was analyzed in HOPE-fixed tumor samples using tissue microarrays (TMAs) and semiquantitative image analysis. The results of both methods were significantly correlated and risk assessment showed an overall concordance of 78% (83/107; high- and low-risk) and of 94% (74/79) regarding only high-risk patients. The data demonstrate that optimized IHC in combination with image analysis can provide adequate clinical significance compared to ELISA-derived determination of uPA and PAI-1.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Middle Aged
Risk Assessment methods
Tissue Array Analysis
Biomarkers, Tumor analysis
Breast Neoplasms chemistry
Carcinoma, Ductal, Breast chemistry
Carcinoma, Lobular chemistry
Image Enhancement
Plasminogen Activator Inhibitor 1 analysis
Urokinase-Type Plasminogen Activator analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1532-3080
- Volume :
- 22
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Breast (Edinburgh, Scotland)
- Publication Type :
- Academic Journal
- Accession number :
- 23332148
- Full Text :
- https://doi.org/10.1016/j.breast.2012.12.011