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Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice.
- Source :
-
PloS one [PLoS One] 2013; Vol. 8 (1), pp. e52972. Date of Electronic Publication: 2013 Jan 09. - Publication Year :
- 2013
-
Abstract
- Weevils can devastate food legumes in developing countries, but genetically modified peas (Pisum sativum), chickpeas and cowpeas expressing the gene for alpha-amylase inhibitor-1 (αAI) from the common bean (Phaseolus vulgaris) are completely protected from weevil destruction. αAI is seed-specific, accumulated at high levels and undergoes post-translational modification as it traverses the seed endomembrane system. This modification was thought to be responsible for the reported allergenicity in mice of the transgenic pea but not the bean. Here, we observed that transgenic αAI peas, chickpeas and cowpeas as well as non-transgenic beans were all allergenic in BALB/c mice. Even consuming non-transgenic peas lacking αAI led to an anti-αAI response due to a cross-reactive response to pea lectin. Our data demonstrate that αAI transgenic peas are not more allergenic than beans or non-transgenic peas in mice. This study illustrates the importance of repeat experiments in independent laboratories and the potential for unexpected cross-reactive allergic responses upon consumption of plant products in mice.
- Subjects :
- Animals
Cicer genetics
Cicer immunology
Cicer metabolism
Cross Reactions immunology
Diet
Epithelium immunology
Epithelium pathology
Fabaceae genetics
Fabaceae immunology
Fabaceae metabolism
Female
Immunization methods
Immunoglobulin E blood
Immunoglobulin E immunology
Immunoglobulin G blood
Immunoglobulin G immunology
Lung immunology
Lung pathology
Mice
Mice, Inbred BALB C
Ovalbumin immunology
Pisum sativum genetics
Pisum sativum metabolism
Plant Lectins genetics
Plant Lectins metabolism
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Pneumonia diagnosis
Pneumonia immunology
Allergens immunology
Pisum sativum immunology
Plant Lectins immunology
Plants, Genetically Modified immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23326368
- Full Text :
- https://doi.org/10.1371/journal.pone.0052972