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Alterations in the MA and NC domains modulate phosphoinositide-dependent plasma membrane localization of the Rous sarcoma virus Gag protein.
- Source :
-
Journal of virology [J Virol] 2013 Mar; Vol. 87 (6), pp. 3609-15. Date of Electronic Publication: 2013 Jan 16. - Publication Year :
- 2013
-
Abstract
- Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P(2)] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P(2) and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P(3)] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P(2) and PI(3,4,5)P(3), suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.
- Subjects :
- Gene Products, gag genetics
Mutant Proteins genetics
Mutant Proteins metabolism
Phosphoproteins genetics
Protein Transport
Viral Matrix Proteins genetics
Cell Membrane metabolism
Gene Products, gag metabolism
Phosphatidylinositols metabolism
Phosphoproteins metabolism
Rous sarcoma virus physiology
Viral Matrix Proteins metabolism
Virus Assembly
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 87
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 23325682
- Full Text :
- https://doi.org/10.1128/JVI.03059-12