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Isolation and characterization of the ecdysone receptor and its heterodimeric partner ultraspiracle through development in Sciara coprophila.
- Source :
-
Chromosoma [Chromosoma] 2013 Mar; Vol. 122 (1-2), pp. 103-19. Date of Electronic Publication: 2013 Jan 16. - Publication Year :
- 2013
-
Abstract
- Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces DNA amplification in Sciara, and the amplification origin of DNA puff II/9A contains a putative binding site for the ecdysone receptor (EcR). We report here the isolation, cloning, and characterizing of two ecdysone receptor isoforms in Sciara (ScEcR-A and ScEcR-B) and the heterodimeric partner, ultraspiracle (ScUSP). ScEcR-A is the predominant isoform in larval tissues and ScEcR-B in adult tissues, contrary to the pattern in Drosophila. Moreover, ScEcR-A is produced at amplification but is absent just prior. We discuss these results in relation to the model of ecdysone regulation of DNA amplification.
- Subjects :
- Amino Acid Sequence
Animals
Binding Sites
Diptera cytology
Drosophila cytology
Drosophila genetics
Larva genetics
Larva metabolism
Protein Binding
Receptors, Steroid isolation & purification
Receptors, Steroid metabolism
Salivary Glands cytology
DNA Replication genetics
Diptera genetics
Ecdysone genetics
Receptors, Steroid genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0886
- Volume :
- 122
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Chromosoma
- Publication Type :
- Academic Journal
- Accession number :
- 23321980
- Full Text :
- https://doi.org/10.1007/s00412-012-0395-4