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Aspirin Induces platelet apoptosis.
- Source :
-
Platelets [Platelets] 2013; Vol. 24 (8), pp. 637-42. Date of Electronic Publication: 2013 Jan 09. - Publication Year :
- 2013
-
Abstract
- Aspirin is widely used in the treatment of a number of clinical conditions. Although aspirin is being thought to be a relatively "safe" medicine, it also has some side effects, particularly the risk of bleeding which may be severe and lead to death. The mechanisms, however, are not totally understood. It has been reported recently that aspirin induces apoptosis in many cell types. Thus, the aim of the current study is to explore whether aspirin induces platelet apoptosis. The data show that mitochondrial transmembrane potential (ΔΨm) depolarizations and phosphatidylserine (PS) exposures were dose-dependently induced by aspirin in platelets. To further confirm that aspirin incurs platelet apoptosis, caspase-3 activity was measured in platelets, and the result indicated that aspirin induced caspase-3 activation. Furthermore, the mean volume of platelets incubated with aspirin was obviously reduced. Caspase inhibitor z-VAD-fmk inhibited aspirin induced apoptotic platelet shrinkage and ΔΨm depolarization, but had no effect on PS exposure. In addition, platelets incubated with cyclooxygenase inhibitor indomethacin did not incur ΔΨm depolarazation and PS exposure. Taken together, the data indicate that aspirin induces platelet apoptosis via caspase-3 activation.
- Subjects :
- Blood Platelets metabolism
Caspase 3 metabolism
Cyclooxygenase Inhibitors pharmacology
Dose-Response Relationship, Drug
Humans
Indomethacin pharmacology
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Mitochondria metabolism
Phosphatidylserines pharmacology
Signal Transduction
Time Factors
Apoptosis drug effects
Aspirin pharmacology
Blood Platelets drug effects
Platelet Aggregation Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1369-1635
- Volume :
- 24
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Platelets
- Publication Type :
- Academic Journal
- Accession number :
- 23301845
- Full Text :
- https://doi.org/10.3109/09537104.2012.754417