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Utility of the bacteriophage RB69 polymerase gp43 as a surrogate enzyme for herpesvirus orthologs.
Utility of the bacteriophage RB69 polymerase gp43 as a surrogate enzyme for herpesvirus orthologs.
- Source :
-
Viruses [Viruses] 2013 Jan 08; Vol. 5 (1), pp. 54-86. Date of Electronic Publication: 2013 Jan 08. - Publication Year :
- 2013
-
Abstract
- Viral polymerases are important targets in drug discovery and development efforts. Most antiviral compounds that are currently approved for treatment of infection with members of the herpesviridae family were shown to inhibit the viral DNA polymerase. However, biochemical studies that shed light on mechanisms of drug action and resistance are hampered primarily due to technical problems associated with enzyme expression and purification. In contrast, the orthologous bacteriophage RB69 polymerase gp43 has been crystallized in various forms and therefore serves as a model system that provides a better understanding of structure-function relationships of polymerases that belong the type B family. This review aims to discuss strengths, limitations, and opportunities of the phage surrogate with emphasis placed on its utility in the discovery and development of anti-herpetic drugs.
- Subjects :
- Amino Acid Sequence
Animals
Bacteriophages chemistry
Bacteriophages genetics
DNA-Directed DNA Polymerase genetics
DNA-Directed DNA Polymerase metabolism
Herpesviridae genetics
Herpesviridae physiology
Humans
Models, Molecular
Nucleic Acid Synthesis Inhibitors
Viral Proteins antagonists & inhibitors
Viral Proteins genetics
Bacteriophages enzymology
DNA-Directed DNA Polymerase chemistry
Herpesviridae enzymology
Herpesviridae Infections virology
Viral Proteins chemistry
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 23299784
- Full Text :
- https://doi.org/10.3390/v5010054