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CD8α+ DCs can be induced in the absence of transcription factors Id2, Nfil3, and Batf3.
- Source :
-
Blood [Blood] 2013 Feb 28; Vol. 121 (9), pp. 1574-83. Date of Electronic Publication: 2013 Jan 07. - Publication Year :
- 2013
-
Abstract
- Antiviral immunity and cross-presentation is mediated constitutively through CD8α+ and CD103+ DCs. Development of these DC subsets is thought to require the transcription factors Irf8, Id2, Nfil3, and Batf3, although how this network is regulated is poorly defined. We addressed the nature of the differentiation blocks observed in the absence of these factors and found that although all 4 factors are required for CD103+ DC development, only Irf8 is essential for CD8α+ DCs. CD8α+ DCs emerged in the absence of Id2, Nfil3 and Batf3 in short-term bone marrow reconstitution. These “induced” CD8α+ DCs exhibit several hallmarks of classic CD8α+ DCs including the expression of CD24, Tlr3, Xcr1, Clec9A, and the capacity to cross-present soluble, cell-associated antigens and viral antigens even in the absence of Batf3. Collectively, these results uncover a previously undescribed pathway by which CD8α+ DCs emerge independent of Id2, Nfil3, and Batf3, but dependent on Irf8.
- Subjects :
- Animals
Antigens, Viral immunology
Basic-Leucine Zipper Transcription Factors genetics
Basic-Leucine Zipper Transcription Factors metabolism
Cell Survival genetics
Cell Survival immunology
Cells, Cultured
Cross-Priming genetics
Cross-Priming immunology
Cross-Priming physiology
Dendritic Cells immunology
Dendritic Cells metabolism
Herpesvirus 1, Human immunology
Inhibitor of Differentiation Protein 2 genetics
Inhibitor of Differentiation Protein 2 metabolism
Interferon Regulatory Factors genetics
Interferon Regulatory Factors metabolism
Interferon Regulatory Factors physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Repressor Proteins genetics
Repressor Proteins metabolism
Transcription Factors genetics
Transcription Factors metabolism
Transcription Factors physiology
Basic-Leucine Zipper Transcription Factors physiology
CD8 Antigens metabolism
Cell Differentiation genetics
Cell Differentiation immunology
Dendritic Cells physiology
Inhibitor of Differentiation Protein 2 physiology
Repressor Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1528-0020
- Volume :
- 121
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 23297132
- Full Text :
- https://doi.org/10.1182/blood-2012-07-445650