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Molecular characterization of a lipoxygenase from the basidiomycete mushroom Pleurotus ostreatus.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2013; Vol. 77 (1), pp. 38-45. Date of Electronic Publication: 2013 Jan 07. - Publication Year :
- 2013
-
Abstract
- The full-length cDNA of the gene PoLOX1 encoding a lipoxygenase (LOX) and its corresponding genomic DNA were isolated from the basidiomycete mushroom Pleurotus ostreatus strain H1. The deduced amino acid sequence of PoLOX1 showed similarity to a valencene dioxygenase of Pleurotus sapidus, putative LOX-like proteins from ascomycete, basidiomycete, and deuteromycete fungi, and known LOXs from plants, animals, and bacteria. PoLOX1 also contained the LOX iron-binding catalytic domain in the C-terminal region, but not the polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain, which is usually found in the N-terminal region of eukaryotic LOXs. Genomic sequence analysis revealed that PoLOX1 was interrupted by one intron, and that the promoter region included TATA and CAAT boxes. Southern blot analysis indicated that PoLOX1 is a member of a small gene family comprising highly similar genes. Northern blot analysis revealed that it is transcribed more abundantly in the stipes of the fruit bodies than in the caps.
- Subjects :
- Amino Acid Sequence
Catalytic Domain
Cloning, Molecular
DNA, Complementary genetics
DNA, Complementary metabolism
Exons
Fruiting Bodies, Fungal genetics
Fungal Proteins classification
Fungal Proteins genetics
Fungal Proteins isolation & purification
Introns
Lipoxygenase classification
Lipoxygenase genetics
Lipoxygenase isolation & purification
Molecular Sequence Data
Phylogeny
Pleurotus genetics
Promoter Regions, Genetic
Sequence Alignment
Sequence Homology, Amino Acid
Fruiting Bodies, Fungal enzymology
Fungal Proteins metabolism
Lipoxygenase metabolism
Pleurotus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 77
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23291746
- Full Text :
- https://doi.org/10.1271/bbb.120484