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Regulation of TLR2-mediated tolerance and cross-tolerance through IRAK4 modulation by miR-132 and miR-212.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2013 Feb 01; Vol. 190 (3), pp. 1250-63. Date of Electronic Publication: 2012 Dec 21. - Publication Year :
- 2013
-
Abstract
- Innate immune response is the first defense against pathogens via recognition by various conserved pattern recognition receptors, such as TLRs, to initiate a rapid and strong cytokine alarm. TLR signaling-mediated cytokine production must be properly regulated to prevent pathological conditions deriving from overproduction of cytokines. In this study, the role of specific microRNAs in TLR-signaling pathway was investigated to reveal the cross-interaction and -regulation in the MyD88 pathway. In peptidoglycan (PGN)/TLR2-stimulated THP-1 monocytes, PBMCs, and primary macrophages showed rapid and dramatic miR-132 and miR-212 (miR-132/-212) upregulation. This newly identified response appeared earlier in time than the characteristic miR-146a response in LPS-TLR4 stimulation. The rapid induction of miR-132/-212 was transcription factor CREB dependent, and the sustained expression of miR-132/-212 was responsible for inducing tolerance to subsequent PGN challenge. Cross-tolerance was observed by TLR5 ligand flagellin and heat-killed or live bacteria resulting from miR-132/-212 upregulation. Mechanistically, IRAK4 was identified and validated as a target of miR-132/-212 by luciferase reporter assay and seed-sequence mutagenesis of the reporter. Transfection of miR-132 or miR-212 alone mimicked PGN tolerance in monocytes, whereas transfected specific miRNA inhibitors tampered the tolerance effect. During bacterial infection, PGN-mediated TLR2 signaling induces miR-132/-212 to downregulate IRAK4, an early component in the MyD88-dependent pathway, whereas LPS/TLR4-induced miR-146a downregulates downstream components of the same MyD88-dependent pathway. The identification of miR-132/-212 and miR-146a together to prevent damaging consequences from the overproduction of proinflammatory cytokines by targeting a common signaling pathway is significant and will provide insights into future design and development of therapeutics.
- Subjects :
- Animals
Bacterial Proteins immunology
Cells, Cultured immunology
Cyclic AMP Response Element-Binding Protein immunology
Female
Flagellin immunology
Flagellin pharmacology
Gene Expression Regulation
Genes, Reporter
Lipopolysaccharides immunology
Lipoproteins pharmacology
Macrophage Activation
Mice
Mice, Inbred C57BL
MicroRNAs antagonists & inhibitors
MicroRNAs biosynthesis
MicroRNAs genetics
Myeloid Differentiation Factor 88 immunology
RNA, Small Interfering pharmacology
Tumor Necrosis Factor-alpha metabolism
Immune Tolerance immunology
Interleukin-1 Receptor-Associated Kinases immunology
Macrophages, Peritoneal immunology
MicroRNAs immunology
Monocytes immunology
Peptidoglycan immunology
Signal Transduction immunology
Toll-Like Receptor 2 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 190
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 23264652
- Full Text :
- https://doi.org/10.4049/jimmunol.1103060