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Effects of β-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis.
- Source :
-
Research in veterinary science [Res Vet Sci] 2013 Jun; Vol. 94 (3), pp. 433-9. Date of Electronic Publication: 2012 Dec 19. - Publication Year :
- 2013
-
Abstract
- An in vitro model was used to investigate effects of β-hydroxybutyrate and isoproterenol (β-adrenergic receptor agonist) on lipolysis in isolated adipocytes from late pregnant and recently calved dairy cows (n=5) and cows with clinical ketosis (n=3). Incubation with 3.0 mmol/L β-hydroxybutyrate reduced lipolysis in isolated adipocytes. This inhibitory effect was lower in the first lactation week (47%±16%) compared with late pregnancy (71%±6.5%). Incubation with 0.3 μmol/L isoproterenol stimulated lipolysis in isolated adipocytes from periparturient dairy cows. Basal lipolysis resulted in non-esterified fatty acid to glycerol ratios in the incubation media of 2.0±0.23 in prepartum samples, 2.1±0.23 in the first lactation week and 2.2±0.09 in cows with clinical ketosis. β-Hydroxybutyrate reduced lipolysis by 45%±9.6% in isolated adipocytes from cows with clinical ketosis, indicating that impaired feedback of β-hydroxybutyrate may not play a role in the disease etiology.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adipocytes metabolism
Animals
Cattle
Dose-Response Relationship, Drug
Female
In Vitro Techniques
Ketosis metabolism
Peripartum Period metabolism
Pregnancy
3-Hydroxybutyric Acid pharmacology
Adipocytes drug effects
Cattle Diseases metabolism
Isoproterenol pharmacology
Ketosis veterinary
Lipolysis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2661
- Volume :
- 94
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Research in veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 23256919
- Full Text :
- https://doi.org/10.1016/j.rvsc.2012.11.009