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3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing.
- Source :
-
Biomaterials [Biomaterials] 2013 Feb; Vol. 34 (7), pp. 1781-9. Date of Electronic Publication: 2012 Dec 08. - Publication Year :
- 2013
-
Abstract
- Although it is expected that hepatocyte-like cells differentiated from human embryonic stem (ES) cells or induced pluripotent stem (iPS) cells will be utilized in drug toxicity testing, the actual applicability of hepatocyte-like cells in this context has not been well examined so far. To generate mature hepatocyte-like cells that would be applicable for drug toxicity testing, we established a hepatocyte differentiation method that employs not only stage-specific transient overexpression of hepatocyte-related transcription factors but also a three-dimensional spheroid culture system using a Nanopillar Plate. We succeeded in establishing protocol that could generate more matured hepatocyte-like cells than our previous protocol. In addition, our hepatocyte-like cells could sensitively predict drug-induced hepatotoxicity, including reactive metabolite-mediated toxicity. In conclusion, our hepatocyte-like cells differentiated from human ES cells or iPS cells have potential to be applied in drug toxicity testing.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 34
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 23228427
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2012.11.029