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Mixed donor chimerism in non-malignant haematological diseases after allogeneic bone marrow transplantation.
- Source :
-
Journal of the College of Physicians and Surgeons--Pakistan : JCPSP [J Coll Physicians Surg Pak] 2012 Dec; Vol. 22 (12), pp. 765-8. - Publication Year :
- 2012
-
Abstract
- Objective: To determine the frequency of mixed donor chimerism in patients of non-malignant haematological diseases after allogeneic bone marrow transplant.<br />Study Design: A cross-sectional, observational study.<br />Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from July 2010 to June 2011.<br />Methodology: Donor chimerism was assessed in patients of aplastic anaemia and beta-thalassaemia major who underwent allogeneic bone marrow transplantation (BMT). Peripheral blood samples were used to assess chimerism status by analysis of short tandem repeats (STR). In patients where pre-transplant blood sample was not available, swab of buccal mucosa was used for pre-transplant STR profile. A standard set of primers for STR markers were used and the amplified DNA was resolved by gel electrophoresis and stained with silver nitrate. The percentage of donor origin DNA was estimated by densitometer.<br />Results: Out of 84 patients, 52 (62%) were males, while 32 (38%) were females. In patients of beta-thalassaemia major, 31 (62%) developed mixed donor chimerism (MC), 13 (26%) developed complete donor chimerism (CC) and 6 (12%) had graft failure. In aplastic anaemia, 17 patients (50%) achieved MC, 13 (38.2%) had CC and 4 (11.8%) developed graft failure. The combined frequency of mixed donor chimerism for both the diseases was 58.3%. D3S1358 was the most informative STR marker in these patients.<br />Conclusion: Majority of the studied patients developed mixed donor chimerism following bone marrow transplantation, whereas only a minor percentage of the patients had graft failure. Analysis of D3S1358 was the most informative in assessing donor chimerism in patients who underwent BMT.
- Subjects :
- Adolescent
Adult
Anemia, Aplastic genetics
Bone Marrow Transplantation adverse effects
Child
Child, Preschool
Chromosome Mapping
Electrophoresis
Female
Gene Amplification
Genetic Markers
Humans
Male
Microsatellite Repeats
Middle Aged
Taq Polymerase
Tissue Donors
Transplantation, Homologous
Young Adult
beta-Thalassemia genetics
Anemia, Aplastic surgery
Bone Marrow Transplantation methods
Chimerism
DNA analysis
Polymerase Chain Reaction methods
beta-Thalassemia surgery
Subjects
Details
- Language :
- English
- ISSN :
- 1681-7168
- Volume :
- 22
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
- Publication Type :
- Academic Journal
- Accession number :
- 23217481
- Full Text :
- https://doi.org/12.2012/JCPSP.765768