Back to Search
Start Over
Proteomic analysis of the Cyanophora paradoxa muroplast provides clues on early events in plastid endosymbiosis.
- Source :
-
Planta [Planta] 2013 Feb; Vol. 237 (2), pp. 637-51. Date of Electronic Publication: 2012 Dec 02. - Publication Year :
- 2013
-
Abstract
- Glaucophytes represent the first lineage of photosynthetic eukaryotes of primary endosymbiotic origin that diverged after plastid establishment. The muroplast of Cyanophora paradoxa represents a primitive plastid that resembles its cyanobacterial ancestor in pigment composition and the presence of a peptidoglycan wall. To attain insights into the evolutionary history of cyanobiont integration and plastid development, it would thus be highly desirable to obtain knowledge on the composition of the glaucophyte plastid proteome. Here, we provide the first proteomic analysis of the muroplast of C. paradoxa. Mass spectrometric analysis of the muroplast proteome identified 510 proteins with high confidence. The protein repertoire of the muroplast revealed novel paths for reduced carbon flow and export to the cytosol through a sugar phosphate transporter of chlamydial origin. We propose that C. paradoxa possesses a primordial plastid mirroring the situation in the early protoalga.
- Subjects :
- Agrobacterium tumefaciens genetics
Agrobacterium tumefaciens metabolism
Carbon metabolism
Cloning, Molecular
Cyanophora genetics
Cytosol metabolism
Phosphate Transport Proteins metabolism
Plant Proteins genetics
Plant Proteins metabolism
Plastids genetics
Protein Transport
Proteomics methods
Protoplasts cytology
Protoplasts metabolism
Tandem Mass Spectrometry
Nicotiana genetics
Nicotiana metabolism
Transformation, Genetic
Biological Evolution
Cyanophora metabolism
Plastids metabolism
Proteome analysis
Symbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2048
- Volume :
- 237
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Planta
- Publication Type :
- Academic Journal
- Accession number :
- 23212214
- Full Text :
- https://doi.org/10.1007/s00425-012-1819-3