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Effects of quercetin on mRNA expression of steroidogenesis genes in primary cultures of Leydig cells treated with atrazine.
- Source :
-
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2013 Mar; Vol. 27 (2), pp. 700-7. Date of Electronic Publication: 2012 Nov 28. - Publication Year :
- 2013
-
Abstract
- The effect of the phytoestrogen, quercetin (QT) on the reproductive toxicity of atrazine (ATZ) was explored in interstitial Leydig cells (ILCs). We measured the mRNA expressions of steroidogenesis genes: steroidogenic acute regulatory protein (StAR), 3β-hydroxysteroid dehydrogenase (3β-HSD), cytochrome-P450 (CYP) 11A1, insulin-like factor 3 (INSL-3), CYP17A1, inhibin-α (INH-α), androgen receptor (AR), estrogen receptor-α (ER-α), and luteinising hormone receptor (LHR) in isolated ILCs by real-time-PCR after cultured cells were treated in vitro with ATZ (232 μM) and QT (50 μM). The mRNA expression of tested genes increased with ATZ treatment and was normalized by quercetin except AR and ER-α expression. Treatment of cells with QT alone (15-50 μM) caused a dose-dependent increase in AR and ER-α mRNA expression. We also found that QT (50 μM) increased the expressions of AR and ER-α in the presence of a sub-threshold level of cyclic-AMP at 1h culture period to the levels seen with maximal stimulation of cyclic-AMP. Furthermore, the expressions of tested genes were unaffected by cyclic-AMP at 6h when the stimulatory effects of ATZ on tested genes were sustained. These findings suggest that ATZ may stimulate the expression of tested steroidogenesis genes in ILCs via a mechanism independent of cyclic-AMP which was partially antagonize by QT.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- 17-Hydroxysteroid Dehydrogenases genetics
Animals
Cells, Cultured
Cholesterol Side-Chain Cleavage Enzyme genetics
Gene Expression Regulation drug effects
Inhibins genetics
Insulin genetics
Leydig Cells metabolism
Male
Phosphoproteins genetics
Proteins genetics
RNA, Messenger metabolism
Rats
Rats, Wistar
Steroid 17-alpha-Hydroxylase genetics
Antioxidants pharmacology
Atrazine toxicity
Herbicides toxicity
Leydig Cells drug effects
Quercetin pharmacology
Receptors, Steroid genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3177
- Volume :
- 27
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Publication Type :
- Academic Journal
- Accession number :
- 23200735
- Full Text :
- https://doi.org/10.1016/j.tiv.2012.11.005