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Macrophage activation by muramyl dipeptide bound to neoglycoproteins and glycosylated polymers: cytotoxic factor production.
- Source :
-
Journal of biological response modifiers [J Biol Response Mod] 1990 Feb; Vol. 9 (1), pp. 33-43. - Publication Year :
- 1990
-
Abstract
- Some biological functions of macrophages can be stimulated by muramyl dipeptide (MDP) in vitro. Such stimulation is more efficient when MDP is bound to macromolecule carriers. The macrophage stimulation by MDP bound to glycosylated serum albumin (BSA) or bound to gluconoylated and glycosylated poly-L-lysine (PLK) is investigated. These two types of MDP conjugates are more efficient than free MDP in rendering mouse peritoneal and rat alveolar macrophages cytostatic against various tumor cells. However, the release of mitogenic factor or cytotoxic factor (CF) by activated macrophages varies according to the nature of the carrier (BSA or PLK) and to the nature and content of sugar residues bound to the macromolecule carrier (mannose or 6-phosphomannose). Macrophages activated by MDP bound to glycosylated BSA release mitogenic factor and CF into the medium; anti-recombinant tumor necrosis factor (rTNF) totally inhibits the cytotoxicity of the supernatant. On the contrary, MDP bound to glycosylated PLK induces no secretion of mitogenic factor and a very small amount of CF in the culture medium. The role of CF in the cytostatic activity of activated macrophages is discussed. The released CF is not involved in the cytostatic activity, but TNF-like molecules, expressed at the membrane level, could be implied because anti-rTNF abrogates 40% of the cytostatic activity of the macrophages.
- Subjects :
- Acetylmuramyl-Alanyl-Isoglutamine pharmacology
Animals
Cells, Cultured
Drug Carriers
Glycoconjugates
Glycoproteins
Mice
Mice, Inbred C3H
Polymers
Rats
Rats, Inbred Strains
Species Specificity
Tumor Necrosis Factor-alpha biosynthesis
Acetylmuramyl-Alanyl-Isoglutamine administration & dosage
Cytotoxins biosynthesis
Macrophage Activation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0732-6580
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biological response modifiers
- Publication Type :
- Academic Journal
- Accession number :
- 2319259