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Th2-Th1 shift with the multiantigenic formulation TERAVAC-HIV-1 in Balb/c mice.
- Source :
-
Immunology letters [Immunol Lett] 2013 Jan; Vol. 149 (1-2), pp. 77-84. Date of Electronic Publication: 2012 Nov 24. - Publication Year :
- 2013
-
Abstract
- In chronic HIV infection a progressive Th1 to Th2/Th0 cytokine-profile shift is related to disease progression. One of the possible benefits of a therapeutic vaccination might be to counterbalance this phenomenon to allow viral replication control under a Th1-type immune response. TERAVAC-HIV-1 is a multiantigenic formulation vaccine candidate against HIV-1 which comprises the recombinant protein CR3 that contains T cell epitopes and the surface and nucleocapsid antigens of Hepatitis B Virus (HBV). Previous studies showed that such virus like particles of the HBV provide a Th1 adjuvant effect. The present studies examined the capacity of TERAVAC to elicit a Th1 response in the presence of an ongoing HIV-specific Th2-type response in Balb/c mice. To examine this issue, we injected subcutaneously the animals with CR3 or viral lysate in alum which resulted in a Th2-type response. The CR3-specific Th2-type response was verified by induction of IL-4 and IL-10 secretion in ex vivo stimulated splenocytes without secretion of IFN-γ and IgG2a antibodies in serum. Further subcutaneous and simultaneous subcutaneous-nasal immunizations of the same mice with TERAVAC promoted IFN-γ secretion and production of IgG2a antibodies in accordance with a Th1-type response. This result suggests a therapeutic benefit of this vaccine candidate in the restoration of the Th1-type HIV-specific cellular response in seropositive patients.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- AIDS Vaccines genetics
AIDS Vaccines immunology
Animals
Cytokines blood
HIV Antibodies blood
HIV-1 genetics
Hepatitis B virus genetics
Hepatitis B virus immunology
Immunoglobulin G blood
Mice
Mice, Inbred BALB C
Nucleocapsid Proteins genetics
Nucleocapsid Proteins immunology
Nucleocapsid Proteins pharmacology
Th1 Cells
Th2 Cells
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Vaccines, Synthetic pharmacology
AIDS Vaccines pharmacology
Cytokines immunology
HIV Antibodies immunology
HIV-1 immunology
Immunoglobulin G immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0542
- Volume :
- 149
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Immunology letters
- Publication Type :
- Academic Journal
- Accession number :
- 23183092
- Full Text :
- https://doi.org/10.1016/j.imlet.2012.11.007