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Th2-Th1 shift with the multiantigenic formulation TERAVAC-HIV-1 in Balb/c mice.

Authors :
García-Díaz D
Rodríguez I
Santisteban Y
Márquez G
Terrero Y
Brown E
Iglesias E
Source :
Immunology letters [Immunol Lett] 2013 Jan; Vol. 149 (1-2), pp. 77-84. Date of Electronic Publication: 2012 Nov 24.
Publication Year :
2013

Abstract

In chronic HIV infection a progressive Th1 to Th2/Th0 cytokine-profile shift is related to disease progression. One of the possible benefits of a therapeutic vaccination might be to counterbalance this phenomenon to allow viral replication control under a Th1-type immune response. TERAVAC-HIV-1 is a multiantigenic formulation vaccine candidate against HIV-1 which comprises the recombinant protein CR3 that contains T cell epitopes and the surface and nucleocapsid antigens of Hepatitis B Virus (HBV). Previous studies showed that such virus like particles of the HBV provide a Th1 adjuvant effect. The present studies examined the capacity of TERAVAC to elicit a Th1 response in the presence of an ongoing HIV-specific Th2-type response in Balb/c mice. To examine this issue, we injected subcutaneously the animals with CR3 or viral lysate in alum which resulted in a Th2-type response. The CR3-specific Th2-type response was verified by induction of IL-4 and IL-10 secretion in ex vivo stimulated splenocytes without secretion of IFN-γ and IgG2a antibodies in serum. Further subcutaneous and simultaneous subcutaneous-nasal immunizations of the same mice with TERAVAC promoted IFN-γ secretion and production of IgG2a antibodies in accordance with a Th1-type response. This result suggests a therapeutic benefit of this vaccine candidate in the restoration of the Th1-type HIV-specific cellular response in seropositive patients.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1879-0542
Volume :
149
Issue :
1-2
Database :
MEDLINE
Journal :
Immunology letters
Publication Type :
Academic Journal
Accession number :
23183092
Full Text :
https://doi.org/10.1016/j.imlet.2012.11.007