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Temporal alteration of spreading depression by the glycine transporter type-1 inhibitors NFPS and Org-24461 in chicken retina.
- Source :
-
Brain research [Brain Res] 2013 Jan 25; Vol. 1492, pp. 1-6. Date of Electronic Publication: 2012 Nov 23. - Publication Year :
- 2013
-
Abstract
- We used isolated chicken retina to induce spreading depression by the glutamate receptor agonist N-methyl-d-aspartate. The N-methyl-d-aspartate-induced latency time of spreading depression was extended by the glycine(B) binding site competitive antagonist 7-chlorokynurenic acid. Addition of the glycine transporter type-1 inhibitors NFPS and Org-24461 reversed the inhibitory effect of 7-chlorokynurenic acid on N-methyl-d-aspartate-evoked spreading depression. The glycine uptake inhibitory activity of Org-24461, NFPS, and some newly synthesized analogs of NFPS was determined in CHO cells stably expressing human glycine transporter type-1b isoform. Compounds, which failed to inhibit glycine transporter type-1, also did not have effect on retinal spreading depression. These experiments indicate that the spreading depression model in chicken retina is a useful in vitro test to determine activity of glycine transporter type-1 inhibitors. In addition, our data serve further evidence for the role of glycine transporter type-1 in retinal neurotransmission and light processing.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
CHO Cells
Chickens
Cortical Spreading Depression drug effects
Cricetinae
Cricetulus
Excitatory Amino Acid Agonists pharmacology
Excitatory Amino Acid Antagonists pharmacology
Humans
Kynurenic Acid analogs & derivatives
Kynurenic Acid pharmacology
N-Methylaspartate pharmacology
Retina drug effects
Sarcosine pharmacology
Cortical Spreading Depression physiology
Glycine Plasma Membrane Transport Proteins antagonists & inhibitors
Retina metabolism
Sarcosine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1872-6240
- Volume :
- 1492
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 23178696
- Full Text :
- https://doi.org/10.1016/j.brainres.2012.11.025