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Prevalence and antimicrobial susceptibility of virulent and avirulent multidrug-resistant Escherichia coli isolated from diarrheic neonatal calves.
- Source :
-
American journal of veterinary research [Am J Vet Res] 2012 Dec; Vol. 73 (12), pp. 1944-50. - Publication Year :
- 2012
-
Abstract
- Objective: To determine the prevalence of selected virulence genes and the antimicrobial susceptibility of multidrug-resistant (MDR) Escherichia coli isolated from diarrheic neonatal calves.<br />Sample: 97 E coli isolates from diarrheic neonatal calves.<br />Procedures: E coli isolates were tested via PCR assay for 6 virulence genes and susceptibility to 17 drugs belonging to 9 classes. A 2-sample test of proportions was used to make comparisons between proportions of virulent and avirulent MDR isolates.<br />Results: 23 of 97 (23.7%) isolates were virulent, and 74 (76.3%) were avirulent. Of the 23 virulent isolates, 15 (65.2%) were positive for K99, 14 (60.9%) for F41, 12 (52.2%) for STa, 9 (39.1%) for Stx1, 6 (26.1%) for intimin, and 0 (0%) for Stx2. Twenty of 23 (87.0%) virulent isolates expressed ≥ 2 virulence genes, and 3 of 23 (13.0%) were positive for 1 virulence factor. Eight of 23 (34.8%) virulent isolates expressed STa, K99, and F41, whereas 1 of 23 (4.4%) was positive for STa, F41, intimin, and Stx1. The second most frequent gene pattern was Stx1 and intimin. Twenty of 23 (87.0%) virulent isolates were MDR; the highest prevalence of resistance was recorded for the macrolide-lincosides, followed by the tetracyclines and penicillins. Also, 17 of 23 (74.0%) virulent isolates were resistant to sulfadimethoxine, and 10 of 23 (43.5%) were resistant to trimethoprim-sulfamethoxazole. Additionally, 60 of 74 (81.0%) avirulent isolates were MDR.<br />Conclusions and Clinical Relevance: The prevalence of multidrug resistance was comparable for virulent and avirulent E coli isolated from diarrheic neonatal calves. Cephalosporins and aminoglycosides had reasonable susceptibility.
- Subjects :
- Animals
Animals, Newborn microbiology
Cattle
Cattle Diseases microbiology
Diarrhea epidemiology
Diarrhea microbiology
Diarrhea veterinary
Escherichia coli genetics
Escherichia coli isolation & purification
Escherichia coli Infections epidemiology
Escherichia coli Infections microbiology
Feces microbiology
Microbial Sensitivity Tests veterinary
Minnesota epidemiology
Multiplex Polymerase Chain Reaction veterinary
North Dakota epidemiology
Prevalence
South Dakota epidemiology
Virulence
Virulence Factors metabolism
Anti-Bacterial Agents pharmacology
Cattle Diseases epidemiology
Drug Resistance, Multiple, Bacterial drug effects
Escherichia coli drug effects
Escherichia coli pathogenicity
Escherichia coli Infections veterinary
Virulence Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1943-5681
- Volume :
- 73
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 23176421
- Full Text :
- https://doi.org/10.2460/ajvr.73.12.1944