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Circulating γ/δ T cells in systemic sclerosis exhibit activated phenotype and enhance gene expression of proalpha2(I) collagen of fibroblasts.
- Source :
-
Journal of dermatological science [J Dermatol Sci] 2013 Jan; Vol. 69 (1), pp. 54-60. Date of Electronic Publication: 2012 Oct 17. - Publication Year :
- 2013
-
Abstract
- Background: Systemic sclerosis (SSc) is a systemic inflammatory and fibrotic disease characterized by activation of circulating T lymphocytes.<br />Objective: To determine phenotypic abnormalities of γ/δ T cells and whether γ/δ T cells influence fibroblasts activation in SSc patients.<br />Methods: Number and proportion of peripheral γ/δ T lymphocytes, and their expressions of cell surface molecules were evaluated by flow cytometry. Isolated γ/δ T cells were cocultured with fibroblast, and mRNA expressions of proalpha1(I) collagen and proalpha2(I) collagen (COL1A2) of fibroblasts were analyzed by real time RT-PCR. γ/δ T cell infiltrations in the skin were examined histopathologically.<br />Results: No significant difference in number and proportion of γ/δ T cells was observed in SSc patients compared to healthy controls (HCs). Geometric mean fluorescence intensity (GMFI) of CD16 and CD69 on γ/δ T cells was significantly increased in patients with diffuse cutaneous SSc (dcSSc) compared to HCs. CD62L expression was significantly decreased in patients with dcSSc compared to HCs. The percentage of CD69 positive γ/δ T cells was significantly higher in patients with SSc than HCs. COL1A2 mRNA expression was significantly higher in fibroblasts cocultured with γ/δ T cells from SSc than that from HCs in cell contact independent manner. Additionally, γ/δ T cell infiltrations were observed in SSc patients' skin.<br />Conclusion: Our results suggest that γ/δ T cells showed activated phenotype in SSc and suggest that SSc γ/δ T cells may play an important role on fibrotic process by upregulation of COL1A2 mRNA expression in fibroblasts.<br /> (Copyright © 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Adult
Aged
Antigens, CD metabolism
Antigens, Differentiation, T-Lymphocyte metabolism
Case-Control Studies
Cells, Cultured
Coculture Techniques
Female
Fibrosis genetics
Humans
L-Selectin metabolism
Lectins, C-Type metabolism
Lymphocyte Count
Male
Middle Aged
Phenotype
RNA, Messenger metabolism
Receptors, Antigen, T-Cell, gamma-delta
Receptors, IgG metabolism
Statistics, Nonparametric
T-Lymphocytes metabolism
Collagen Type I biosynthesis
Collagen Type I genetics
Fibroblasts metabolism
Scleroderma, Systemic genetics
Scleroderma, Systemic pathology
T-Lymphocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-569X
- Volume :
- 69
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of dermatological science
- Publication Type :
- Academic Journal
- Accession number :
- 23159281
- Full Text :
- https://doi.org/10.1016/j.jdermsci.2012.10.003