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Are gastric cancer resection margin proteomic profiles more similar to those from controls or tumors?
- Source :
-
Journal of proteome research [J Proteome Res] 2012 Dec 07; Vol. 11 (12), pp. 5836-42. Date of Electronic Publication: 2012 Nov 13. - Publication Year :
- 2012
-
Abstract
- A strategy for treating cancer is to surgically remove the tumor together with a portion of apparently healthy tissue surrounding it, the so-called "resection margin", to minimize recurrence. Here, we investigate whether the proteomic profiles from biopsies of gastric cancer resection margins are indeed more similar to those from healthy tissue than from cancer biopsies. To this end, we analyzed biopsies using an offline MudPIT shotgun proteomic approach and performed label-free quantitation through a distributed normalized spectral abundance factor approach adapted for extracted ion chromatograms (XICs). A multidimensional scaling analysis revealed that each of those tissue-types is very distinct from each other. The resection margin presented several proteins previously correlated with cancer, but also other overexpressed proteins that may be related to tumor nourishment and metastasis, such as collagen alpha-1, ceruloplasmin, calpastatin, and E-cadherin. We argue that the resection margin plays a key role in Paget's "soil to seed" hypothesis, that is, that cancer cells require a special microenvironment to nourish and that understanding it could ultimately lead to more effective treatments.
- Subjects :
- Biomarkers, Tumor metabolism
Biopsy
Cadherins metabolism
Case-Control Studies
Ceruloplasmin metabolism
Chromatography, Ion Exchange methods
Collagen Type XI metabolism
Databases, Protein
Female
Humans
Male
Neoplasm Metastasis diagnosis
Neoplasm Proteins metabolism
Prognosis
Proteomics methods
Pyloric Antrum metabolism
Pyloric Antrum pathology
Stomach Neoplasms diagnosis
Stomach Neoplasms pathology
Biomarkers, Tumor analysis
Proteome analysis
Software
Stomach Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 11
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 23145836
- Full Text :
- https://doi.org/10.1021/pr300612x