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Using FRET to study RanGTP gradients in live mouse oocytes.

Authors :
Dumont J
Verlhac MH
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 957, pp. 107-20.
Publication Year :
2013

Abstract

Oocytes are extremely large cells that have to coordinate accurate chromosome segregation, asymmetric cytoplasm partitioning together with their own development as fertilizable gametes. For this, they undergo both global (cell cycle progression related) and local changes. It is therefore essential to be able to monitor local changes as they take place in live maturing oocytes. We describe here a method to follow RanGTP gradients using FRET technology in vivo.

Subjects

Subjects :
Animals
Cell Separation
Cell Survival
Female
Mice
Molecular Imaging
Ovary cytology
RNA, Complementary genetics
ran GTP-Binding Protein genetics
Fluorescence Resonance Energy Transfer methods
Oocytes cytology
Oocytes metabolism
ran GTP-Binding Protein metabolism

Details

Language :
English
ISSN :
1940-6029
Volume :
957
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
23138947
Full Text :
https://doi.org/10.1007/978-1-62703-191-2_7
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