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Inhibitory effects of wasabi isothiocyanates on chemical mediator release in RBL-2H3 rat basophilic leukemia cells.
- Source :
-
Journal of nutritional science and vitaminology [J Nutr Sci Vitaminol (Tokyo)] 2012; Vol. 58 (4), pp. 303-7. - Publication Year :
- 2012
-
Abstract
- Wasabi is a plant of Japanese origin. It belongs to the family Brassicaceae and produces various isothiocyanates (ITCs). To clarify the type I allergies inhibited by wasabi ITCs, we investigated the inhibitory effect on chemical mediator release from dinitrophenylated bovine serum albumin (DNP-BSA)-stimulated RBL-2H3 rat basophilic leukemia cells. Allyl ITC (AITC), sec-butyl ITC (s-BuITC), and 3-butenyl ITC (3-BuITC), which have 3 or 4 carbon chains, inhibited histamine release but did not inhibit the release of leukotriene B4 (LTB4) or cysteinyl LTs (CysLTs). 4-Pentenyl ITC (4-PeITC) and 5-hexenyl ITC (5-HeITC), which have 5 or 6 carbon chains and an unsaturated bond at the end, inhibited LTB4 release but did not inhibit the release of histamine or CysLTs. 6-Methylthiohexyl ITC (6-MTITC), 6-methylsulfinylhexyl ITC (6-MSITC), and 6-methylsulfonylhexyl ITC (6-MSFITC), which have a sulfur atom inserted at the end of a 6-carbon chain, inhibited the release of histamine, LTB4, and CysLTs and the elevation in intracellular Ca(2+). These results suggest that wasabi ITCs inhibited type I allergies by inhibiting chemical mediator release and that the inhibitory effects on each chemical mediator were due to differences in the side chain structure of the wasabi ITCs.
- Subjects :
- Animals
Cattle
Cell Line, Tumor
Dinitrophenols metabolism
Histamine Release drug effects
Leukemia, Basophilic, Acute pathology
Leukotriene B4 antagonists & inhibitors
Leukotriene B4 metabolism
Rats
Serum Albumin, Bovine metabolism
Isothiocyanates pharmacology
Plant Extracts pharmacology
Wasabia chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1881-7742
- Volume :
- 58
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of nutritional science and vitaminology
- Publication Type :
- Academic Journal
- Accession number :
- 23132316
- Full Text :
- https://doi.org/10.3177/jnsv.58.303