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Strong correlation between tax and HBZ mRNA expression in HAM/TSP patients: distinct markers for the neurologic disease.
- Source :
-
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2013 Feb; Vol. 56 (2), pp. 135-40. Date of Electronic Publication: 2012 Nov 03. - Publication Year :
- 2013
-
Abstract
- Background: HTLV-1 proviral load is a risk marker for HAM/TSP, but it is insufficient to determine the disease outcome. HTLV-1 Tax and HBZ proteins have been implicated in HAM/TSP pathogenesis in inducing cell proliferation and cytotoxic T lymphocytes response.<br />Objectives: To quantify the expression of tax and HBZ mRNA in asymptomatic carriers (AC) and HAM patients, and to investigate their association with HAM/TSP.<br />Study Design: We quantified the expression of HTLV-1 tax and HBZ mRNA in 37 AC and 26 HAM patients classified according to proviral load as low (AC(L) and HAM(L): <1% infected cells) or high (AC(H) and HAM(H): >1%).<br />Results: The AC(L) subgroup showed the lowest frequency of individuals expressing tax mRNA in comparison with AC(H), HAM(L) and HAM(H), and tax mRNA load normalized by proviral load was significantly lower in the AC(L). In turn, normalized HBZ mRNA expression was similar in all subgroups. Both tax and HBZ mRNA expression were moderately correlated with proviral load in AC (r=0.6, p<0.001) and were weaker in HAM (r=0.4, p<0.05). In contrast, the correlation between tax and HBZ mRNA load was moderate in AC (r=0.5, p=0.001) and was much stronger in HAM (r=0.8, p<0.001). In addition, HBZ mRNA load, but not tax, was significantly associated with motor disability in HAM patients (p=0.036).<br />Conclusions: The expression of tax mRNA seems to be best to estimate the risk of HAM/TSP, whereas HBZ mRNA appears to be a surrogate marker to disease progression, indicating that they have important but distinct roles in HAM/TSP pathogenesis.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Biomarkers
Carrier State virology
Gene Expression Profiling
Human T-lymphotropic virus 1 genetics
Humans
Prognosis
Retroviridae Proteins
Virulence Factors biosynthesis
Basic-Leucine Zipper Transcription Factors biosynthesis
Gene Expression
Gene Products, tax biosynthesis
HTLV-I Infections pathology
HTLV-I Infections virology
Human T-lymphotropic virus 1 pathogenicity
RNA, Messenger biosynthesis
Viral Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5967
- Volume :
- 56
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 23127563
- Full Text :
- https://doi.org/10.1016/j.jcv.2012.10.003