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Comparable cell survival between high dose rate flattening filter free and conventional dose rate irradiation.
- Source :
-
Acta oncologica (Stockholm, Sweden) [Acta Oncol] 2013 Apr; Vol. 52 (3), pp. 652-7. Date of Electronic Publication: 2012 Nov 06. - Publication Year :
- 2013
-
Abstract
- Purpose: Investigation of clonogenic cell survival and cell proliferation following single dose and fractionated delivery of high dose rate flattening filter free (FFF) irradiation compared to conventional dose rates.<br />Material and Methods: The human astrocytoma D384, glioma T98 and lung carcinoma SW1573 cell lines were irradiated using either a single dose (0-12 Gy) or a fractionated protocol of 5 daily fractions of 2 Gy (D384) or 3 Gy (SW1573). Cells were irradiated inside a phantom using fixed gantry beams of a linear accelerator. A sliding window technique created homogeneous dose distributions over the surface of the cell cultures. Irradiations using standard beams (6 MV, 600 MU/min.) and high dose rate FFF beams (10 MV, 2400 MU/min.) were compared. Cell survival was determined by clonogenic assay. In the fractionated irradiation set-up, the number of clonogenic cells was estimated by including tumor cell proliferation during the overall treatment time in the analysis.<br />Results: All cell lines showed equal cell survival following irradiation using either the FFF beams or conventional flattened (FF) beams. This was observed after single dose exposure (0-12 Gy) as well as after fractionated irradiation (p = 0.08 for D384 and 0.20 for SW1373 cell lines).<br />Conclusion: FFF irradiation with a dose rate of 2400 MU/min and four times higher dose per pulse compared to irradiation with FF beams did not change cell survival for three human cancer cell lines up to a fraction dose of 12 Gy compared to irradiation using FF beams.
- Subjects :
- Cell Line, Tumor
Cell Survival radiation effects
Filtration instrumentation
Humans
Models, Biological
Neoplasms pathology
Phantoms, Imaging
Radiotherapy Dosage
Radiotherapy Planning, Computer-Assisted instrumentation
Radiotherapy Planning, Computer-Assisted methods
Radiotherapy, Intensity-Modulated instrumentation
Tomography, X-Ray Computed
Tumor Stem Cell Assay
Cell Proliferation radiation effects
Dose-Response Relationship, Radiation
Filtration methods
Neoplasms radiotherapy
Radiotherapy, Intensity-Modulated methods
Subjects
Details
- Language :
- English
- ISSN :
- 1651-226X
- Volume :
- 52
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Acta oncologica (Stockholm, Sweden)
- Publication Type :
- Academic Journal
- Accession number :
- 23126524
- Full Text :
- https://doi.org/10.3109/0284186X.2012.737021