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Indigenous and spoilage microbiota of farmed sea bream stored in ice identified by phenotypic and 16S rRNA gene analysis.
- Source :
-
Food microbiology [Food Microbiol] 2013 Feb; Vol. 33 (1), pp. 85-9. Date of Electronic Publication: 2012 Sep 13. - Publication Year :
- 2013
-
Abstract
- Investigation of the initial and spoilage microbial diversity of iced stored sea bream was carried out. Culture dependent methods were used for bacterial enumeration and phenotypic identification of bacterial isolates, while culture independent methods, using bacterial 16S rRNA gene amplification, cloning and sequencing of DNA extracted directly from the flesh were also employed. The culture dependent approach revealed that the initial microbiota was dominated by Acinetobacter, Shewanella, Pseudomonas and Flavobacterium, while at the end of shelf-life determined by sensory analysis (16 days), the predominant microbiota was Pseudomonas and Shewanella. Culture independent approach showed that initially the sea bream flesh was strongly dominated by Acinetobacter, while Pseudomonas, Aeromonas salmonicida and Shewanella were the predominant phylotypes at the end of shelf-life. Initial and spoilage microbiota comprised of phylotypes previously identified by others using traditional or molecular techniques. However, Aeromonas has not been reported as part of the dominant microbiota of sea bream at the time of spoilage. Combination of classical and molecular methodologies better reveals the microbiota during storage by revealing bacteria that escape standard approaches and, thus, provides valuable complementary information regarding microbiological spoilage.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacteria classification
Bacteria genetics
DNA, Bacterial genetics
Fisheries
Food Contamination analysis
Food Preservation
Molecular Sequence Data
Phenotype
Phylogeny
Bacteria isolation & purification
Bacterial Typing Techniques methods
Metagenome
RNA, Ribosomal, 16S genetics
Sea Bream microbiology
Seafood microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9998
- Volume :
- 33
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 23122505
- Full Text :
- https://doi.org/10.1016/j.fm.2012.09.001