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[Effect of osteogenically and adipogenically differentiated bone mesenchymal stem cells from mouse on osteoclast formation].

Authors :
Zhu H
Liu YL
Chen JD
Li H
Liu YX
Xu FF
Jiang XX
Zhang Y
Mao N
Source :
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2012 Oct; Vol. 20 (5), pp. 1187-90.
Publication Year :
2012

Abstract

This study was purposed to investigate the regulatory effects of differentiating mesenchymal stem cells (MSC) on osteoclast formation. The MSC from mouse compact bones were cultured and induced into osteoblasts and adipocytes for one week. To test their regulatory effect on osteoclastogenesis, osteogenically differentiated and adipogenically differentiated MSC were co-cultured with CD11b(+) monocytes and osteoclasts were identified with in situ tartrate-resistant acid phosphatase (TRAP) staining. The results showed that differentiated MSC supported osteoclastogenesis but the osteoclast supporting capacity of osteogenically differentiated MSC decreased as compared with undifferentiated MSC. More interestingly, the adipogenically differentiated MSC significantly promoted osteoclasts formation when co-cultured with monocytes. It is concluded that the regulatory effect of MSC on osteoclast formation has changed while they have differentiated into different types of cells. The findings indicate that MSC may exert alternative effect on osteoclastogenesis by differentiation to descendant cells.

Details

Language :
Chinese
ISSN :
1009-2137
Volume :
20
Issue :
5
Database :
MEDLINE
Journal :
Zhongguo shi yan xue ye xue za zhi
Publication Type :
Academic Journal
Accession number :
23114145