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Demonstration of early functional compromise of bone marrow derived hematopoietic progenitor cells during bovine neonatal pancytopenia through in vitro culture of bone marrow biopsies.
- Source :
-
BMC research notes [BMC Res Notes] 2012 Oct 30; Vol. 5, pp. 599. Date of Electronic Publication: 2012 Oct 30. - Publication Year :
- 2012
-
Abstract
- Background: Bovine neonatal pancytopenia (BNP) is a syndrome characterised by thrombocytopenia associated with marked bone marrow destruction in calves, widely reported since 2007 in several European countries and since 2011 in New Zealand. The disease is epidemiologically associated with the use of an inactivated bovine virus diarrhoea (BVD) vaccine and is currently considered to be caused by absorption of colostral antibody produced by some vaccinated cows ("BNP dams"). Alloantibodies capable of binding to the leukocyte surface have been detected in BNP dams and antibodies recognising bovine MHC class I and β-2-microglobulin have been detected in vaccinated cattle. In this study, calves were challenged with pooled colostrum collected from BNP dams or from non-BNP dams and their bone marrow hematopoietic progenitor cells (HPC) cultured in vitro from sternal biopsies taken at 24 hours and 6 days post-challenge.<br />Results: Clonogenic assay demonstrated that CFU-GEMM (colony forming unit-granulocyte/erythroid/macrophage/megakaryocyte; pluripotential progenitor cell) colony development was compromised from HPCs harvested as early as 24 hour post-challenge. By 6 days post challenge, HPCs harvested from challenged calves failed to develop CFU-E (erythroid) colonies and the development of both CFU-GEMM and CFU-GM (granulocyte/macrophage) was markedly reduced.<br />Conclusion: This study suggests that the bone marrow pathology and clinical signs associated with BNP are related to an insult which compromises the pluripotential progenitor cell within the first 24 hours of life but that this does not initially include all cell types.
- Subjects :
- Animals
Animals, Newborn
Biomarkers metabolism
Biopsy
Cattle
Cell Proliferation
Cell Shape
Cells, Cultured
Colony-Forming Units Assay
Colostrum immunology
Diarrhea Viruses, Bovine Viral immunology
Female
Hematopoietic Stem Cells immunology
Hematopoietic Stem Cells metabolism
Male
Pancytopenia immunology
Pancytopenia metabolism
Pilot Projects
Pluripotent Stem Cells immunology
Pluripotent Stem Cells metabolism
Pregnancy
Syndrome
Thrombocytopenia immunology
Thrombocytopenia metabolism
Time Factors
Vaccination
Vaccines immunology
Hematopoietic Stem Cells pathology
Pancytopenia pathology
Pluripotent Stem Cells pathology
Thrombocytopenia pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1756-0500
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- BMC research notes
- Publication Type :
- Academic Journal
- Accession number :
- 23110710
- Full Text :
- https://doi.org/10.1186/1756-0500-5-599