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Association of acute phase protein-haptoglobin, and epithelial-mesenchymal transition in buccal cancer: a preliminary report.
- Source :
-
Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2013 Feb; Vol. 51 (2), pp. 429-37. - Publication Year :
- 2013
-
Abstract
- Background: The aim of this study was to determine the influence of inflammation on acute phase protein and epithelial-mesenchymal transition (EMT) in buccal cancer.<br />Methods: Western blotting was carried out to investigate the expression of haptoglobin and epithelial-mesenchymal transition in oral cancer cell lines with or without IL-6 stimulation. We studied patients with buccal cancer patients without distant metastasis at diagnosis. Correlation between cellular haptoglobin, EMT, and clinical characteristics of buccal cancer was analyzed to assess the prognostic value of cellular haptoglobin level and EMT. The relationship of haptoglobin, and EMT expression with survival was assessed using Cox proportional hazard models.<br />Results: Western blotting analysis showed that increased haptoglobin protein was associated with overexpression of vimentin. Under IL-6 stimulation, overexpression of haptoglobin, EMT-associated motile phenotype was noted in OC2 cell lines. Overexpression of haptoglobin was also associated with an increased risk for locoregional recurrence [hazard ratio (HR) 1.04; p=0.011] after adjusting for age, gender, disease site, stage, and treatment modality.<br />Conclusions: Increased cellular expression of haptoglobin is associated with EMT in oral cancer cell lines and this phenomenon could be exaggerated with IL-6. Cellular expression of haptoglobin is related to locoregional recurrence rate in buccal cancer patients.
- Subjects :
- Adult
Aged
Aged, 80 and over
Cell Line, Tumor
Cell Movement
Cheek pathology
Female
Humans
Immunohistochemistry
Interleukin-6 pharmacology
Male
Middle Aged
Proportional Hazards Models
Vimentin biosynthesis
Epithelial-Mesenchymal Transition
Haptoglobins biosynthesis
Mouth Neoplasms metabolism
Mouth Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1437-4331
- Volume :
- 51
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical chemistry and laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 23093274
- Full Text :
- https://doi.org/10.1515/cclm-2012-0197